Gene Target Identification
Gene Target Identification - Related Videos
08:56
Recombineering and Gene Targeting
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2023
One of the most widely used tools in modern biology is molecular cloning with restriction enzymes, which create compatible ends between DNA fragments that allow them to be joined together. However, this technique has certain restrictions that limit its applicability for large or complex DNA construct generation. A newer technique that addresses some of these shortcomings is recombineering, which modifies DNA using homologous recombination (HR), the exchange between different DNA molecules based...
01:29
Pharmacogenomics: Identification of New Drug Targets
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2026
Advances in genomics have profoundly influenced drug discovery by increasing both the speed and accuracy of pharmaceutical development. Pharmacogenomics, which examines how genetic variation influences drug response, facilitates the identification of novel therapeutic targets and enables patient stratification for personalized treatment. These strategies contribute to improved drug efficacy, minimized adverse effects, and more efficient clinical trial design.Mapping genetic differences...
08:17
VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
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Cited by 22 •
2013
Virus-induced gene silencing is an useful tool for identifying genes involved in nonhost resistance of plants. We demonstrate the use of bacterial pathogens expressing GFPuv in identifying gene silenced plants susceptible to nonhost pathogens. This approach is easy, fast and facilitates large scale screening and similar protocol can be applied to studying various other plant-microbe interactions.
08:48
Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
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Cited by 5 •
2017
A strategy for generating mutations in histone genes at their endogenous location in Saccharomyces cerevisiae is presented.
05:33
Biolistic-Mediated Gene Transfer: A Technique to Deliver Gene of Interest in Target Cells via a Biolistic Gene Gun
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2025
In this video, we demonstrate the protocol of biolistic gene gun-mediated gene delivery in cultured cells. This method provides a simple method of stable genetic transformation using a minimal quantity of DNA.
FAQs
Association alone does not establish that a gene influences the observed outcome. Researchers prioritize candidates when multiple evidence types converge, such as genomic or transcriptomic differences, genetic-screen results, expression patterns, and pathway relationships. They then examine whether altering the candidate changes the relevant phenotype, providing stronger support for a causal role than correlation by itself.
Each evidence type captures a different relationship between genes and biological outcomes. Genomic comparisons can highlight genetic differences, transcriptomic analysis can reveal altered expression, and pathway relationships can place candidates within connected biological processes. Combining these perspectives helps researchers prioritize genes supported by complementary observations rather than relying on a single molecular measurement.
These experiments test whether changing a candidate produces a corresponding change in the phenotype of interest. Gene editing can alter the gene, while knockdown reduces its activity. Observing the resulting biological response helps evaluate whether the candidate contributes functionally to a disease pathway, trait, or intervention response, moving the investigation beyond descriptive molecular patterns.
Pathway relationships show how a candidate may connect with other genes or gene products involved in a biological process. This context can help researchers interpret why the candidate is linked to a trait, disease pathway, or treatment response. It also supports prioritization by indicating whether the candidate fits a broader molecular mechanism identified through other evidence.
A typical workflow begins by comparing genomic or transcriptomic data, examining expression patterns, reviewing genetic-screen findings, and considering pathway relationships. Researchers use these results to prioritize candidate genes or regulatory elements. They then alter selected candidates through gene editing or knockdown and assess whether the relevant phenotype changes, using the outcome to refine the target list.
The approach is useful when researchers need to connect molecular changes with observable biological outcomes. In studies of development, disease, or cellular function, it can help identify genes or regulatory elements associated with important traits and pathways. The resulting candidates may also support biomarker discovery or inform the search for precisely directed therapies.
A well-supported target can clarify how molecular mechanisms contribute to a phenotype or disease pathway. It may reveal a biomarker associated with a biological state, strengthen understanding of cellular function, or identify a point for further intervention studies. These outcomes help translate genomic and functional evidence into testable biological explanations and more focused research directions.