Heart Wall Layers

Heart wall layers are the three tissue layers that form the wall of the heart and enable it to contain blood, contract, and maintain smooth internal flow. From outer to inner, the epicardium provides an external covering, the myocardium contains cardiac muscle that contracts to pump blood, and the endocardium lines the chambers and valves with a smooth surface. Their coordinated structure supports pressure generation, prevents excessive friction within the chambers, and helps separate the heart’s pumping function from surrounding tissues. Understanding these layers is essential in biology for interpreting cardiac development, circulation, and conditions such as inflammation, muscle damage, and structural heart disease.

Heart Wall Layers - Related Videos

Education

JoVE Core - Anatomy and Physiology

Layers of the Heart Wall

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2025

The heart wall comprises three distinct layers: the epicardium, myocardium, and endocardium. The outermost layer, the epicardium, is the visceral layer of the serous pericardium, featuring a thin, transparent mesothelial surface and an inner layer of areolar connective tissue with fat deposits that increase with age. The myocardium, the thickest layer, consists of cardiac muscle cells interconnected by intercalated discs and crisscrossing connective tissue fibers. These muscle fibers contract...

Research

JoVE Journal - Medicine
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Reduction in Left Ventricular Wall Stress and Improvement in Function in Failing Hearts using Algisyl-LVR

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Cited by 34 •

2013

This article describes procedures for implanting a novel hydrogel in failing hearts and quantifying its effect on left ventricular wall stress and function. These procedures have been successfully applied in dogs and humans.

A Novel Microsurgical Model for Heterotopic, En Bloc Chest Wall, Thymus, and Heart Transplantation in Mice

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Cited by 7 •

2016

To study combined solid organ and vascularized composite allotransplantation, we describe a novel heterotopic en bloc chest wall, thymus, and heart transplant model in mice using a cervical non-suture cuff technique.

Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart

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Cited by 13 •

2020

We present a protocol for the isolation of endocardial and coronary endothelial cells from rat hearts through sequential tissue digestion in a digestion buffer, cell collection from recurrent centrifuge cycles, and cell purification using anti-rat CD31 microbeads.

Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall

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Cited by 17 •

2015

Advancements in biomaterial technologies enable the development of three-dimensional multi-cell-type constructs. We have developed electrospinning protocols to produce three individual scaffolds to culture the main structural cells of the airway to provide a 3D in vitro model of the airway bronchiole wall.

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