Heme Extraction
Heme Extraction - Related Videos
09:43
Measurement of Heme Synthesis Levels in Mammalian Cells
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Cited by 12 •
2015
Altered intracellular heme levels are associated with common diseases such as cancer. Thus, there is a need to measure heme biosynthesis levels in diverse cells. The goal of this protocol is to provide a fast and sensitive method to measure and compare the levels of heme synthesis in different cells.
03:03
Bathophenanthroline Sulfonate-Based Colorimetric Assay: A Simple and Rapid Method for Quantitation of Non-Heme Iron in Mouse Liver Tissue
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2025
In this video, we describe a colorimetric-based method to determine non-heme iron content in mouse liver tissue. Bathophenanthroline sulfonate is used as the chromogenic substrate in the presence of a reducing agent, which converts ferric iron to chelatable ferrous iron.
06:39
Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure
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Cited by 40 •
2009
Gua Sha, traditional Chinese therapeutic skin scraping, causes subcutaneous microvascular blood extravasation. We report a protocol of bioluminescence imaging of HO-1-luciferase transgenic mice to demonstrate that Gua Sha upregulates heme oxygenase-1 (HO-1) in multiple organs.
02:55
Extraction - Concepts
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2020
Extraction Extraction is a common technique used in organic chemistry to isolate a target compound. In the extraction process, a solute is transferred from one phase to another to separate it from unreacted starting materials or impurities. Extraction is also used to facilitate the isolation of a solute from a reaction solvent that is difficult to remove by evaporation, such as a solvent with a high boiling point. Generally, there are three types of extractions. First, in solid-liquid...
06:42
Extraction of Biomarkers from Sediments - Accelerated Solvent Extraction
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst The distribution of a group of organic biomarkers called glycerol-dialkyl glycerol-tetraethers (GDGTs), produced by a suite of archaea and bacteria, were found in modern sediments to change in a predictable manner in response to air or water temperature1,2. Therefore, the distribution of these biomarkers in a sequence of sediments of known age can be used to reconstruct the evolution of air and/or water temperature on...
FAQs
Denaturation separates the globin component from the heme-containing portion of the sample. Once the surrounding protein structure is disrupted, the relatively hydrophobic heme can be separated more effectively from other biomolecules under acidic or organic-solvent conditions. This step is therefore central to reducing interference from globin during later biochemical analysis.
Heme’s relative hydrophobicity provides a chemical basis for separating it from many surrounding biological molecules. After cells or proteins are disrupted, extraction conditions can take advantage of this property to isolate heme from the denatured protein material. The resulting separation supports more focused measurements of heme content and examination of heme-associated biological systems.
Acidic conditions or organic solvents provide the separation environment used after the sample has been disrupted and the globin component denatured. Their purpose is to help separate the relatively hydrophobic heme from surrounding biomolecules. Selecting an appropriate condition can support recovery of heme in a form suitable for subsequent purification and biochemical analysis.
A typical workflow begins with disruption of cells or proteins, followed by denaturation of the globin component. The sample then undergoes separation under acidic conditions or with organic solvents, allowing heme to be isolated from other biomolecules. Subsequent purification can produce a cleaner preparation while preserving the heme’s characteristic color and iron center.
Extracted heme supports measurements of heme content and enables biochemical analysis of the isolated material. Researchers can also use it to examine hemoglobin structure and turnover or investigate proteins that bind heme. Preserving the characteristic color and iron center during purification helps maintain features relevant to these analyses.
In biology, the method connects molecular measurements with several heme-related processes. It can support studies of hemoglobin, heme-binding proteins, and heme turnover, while also contributing to analyses of oxygen transport, cellular respiration, and porphyrin metabolism. These applications make extraction useful for examining how heme participates in proteins and broader cellular pathways.