Hev Nanoparticles
Hev Nanoparticles - Related Videos
09:12
Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods
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Cited by 19 •
2018
We have engineered the capsid protein of hepatitis E virus as a theranostic nanoparticle (HEVNP). HEVNP self-assembles into a stable icosahedral cage in mucosal delivery. Here, we describe the modification of HEVNPs for tumor targeting by mutating surface-exposed residues to cysteines, which conjugate synthetic ligands that specifically bind tumor cells.
03:24
Visualization of Hepatitis E Virus Nanoparticles by Transmission Electron Microscopy
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2026
Source: Chen, C. C., et al. Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods. J. Vis. Exp. (2018)This video demonstrates the preparation and negative staining of hepatitis E virus-like nanoparticles for visualization by transmission electron microscopy. The method highlights the structural integrity of self-assembled capsid-only particles, confirming their uniformity and purity through high-resolution imaging.
09:23
Harmonic Nanoparticles for Regenerative Research
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Cited by 1 •
2014
Protocol details are provided for in vitro labeling human embryonic stem cells with second harmonic generating nanoparticles. Methodologies for hESC investigation by multi-photon microscopy and their differentiation into cardiac clusters are also presented.
13:39
Optical Trapping of Nanoparticles
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Cited by 7 •
2013
The following setup approach details low power optical trapping of dielectric nanoparticles using a double-nanohole in metal film.
07:40
Production of Metal Nanoparticles by Pulsed Laser-ablation in Liquids: A Tool for Studying the Antibacterial Properties of Nanoparticles
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Cited by 14 •
2017
The antimicrobial properties of metals such as copper and silver have been recognized for centuries. This protocol describes pulsed laser-ablation in liquids, a method of synthesizing metal nanoparticles that provides the ability to fine tune the properties of these nanoparticles to optimize their antimicrobial effects.
FAQs
Their self-assembly produces ordered particles that reproduce important architectural and surface characteristics of the HEV capsid. This organization matters because viral structure is not examined only as isolated protein; the assembled form can display antigenic features in a more biologically relevant arrangement. Researchers can therefore investigate how particle architecture relates to host recognition and immune responses.
Without the viral genome required for replication, the particles can present selected HEV surface features without producing infectious virus. This distinction allows researchers to examine antigen recognition and antibody responses while avoiding the replication process associated with complete virus. The resulting system is useful for evaluating viral biology and vaccine concepts in a safer experimental format.
Hev Nanoparticles retain relevant structural and antigenic features of the viral surface, but they do not contain the genome needed for replication. Complete HEV provides the context of a whole virus, whereas these assemblies focus attention on capsid architecture and immune recognition. That focused comparison helps researchers study antibody responses and vaccine candidates without relying on infectious virus.
Researchers can use these assemblies to examine viral architecture, host recognition, and antibody responses. Their ordered capsid-protein structure provides a model for studying how HEV surface features are organized and perceived by the immune system. Findings from these analyses can support structural biology and help identify vaccine candidates that preserve relevant antigenic characteristics.
In vaccine research, the particles provide a way to evaluate HEV antigenic structures without producing infectious virus. Researchers can investigate whether the displayed surface features are suitable for generating or studying antibody responses, then use those observations to inform candidate design. Their genome-free format also supports exploration of safer antigen-delivery platforms.
HEV nanoparticles offer an assembled model of the viral capsid for investigating viral architecture and surface organization. Structural observations can clarify how HEV features are arranged, while immune studies can reveal how hosts and antibodies recognize those features. Together, these uses may guide vaccine development, safer antigen delivery, and broader antiviral research focused on HEV.