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Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods
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Chapters
- 00:04Title
- 00:50HEVNP Characterization
- 02:46Chemical Conjugation of HEVNPs with Biotin, Cancer Targeting Ligand, and Fluorophores
- 07:05Results: Production of LXY30-HEVNPs and Binding/Internalization of Fluorescently Labeled LXY30-HEVNPs in MDA-MB-231 Breast Cancer Cells
- 08:35Conclusion
We have engineered the capsid protein of hepatitis E virus as a theranostic nanoparticle (HEVNP). HEVNP self-assembles into a stable icosahedral cage in mucosal delivery. Here, we describe the modification of HEVNPs for tumor targeting by mutating surface-exposed residues to cysteines, which conjugate synthetic ligands that specifically bind tumor cells.
