Hexane Extraction
Hexane Extraction - Related Videos
09:32
Solid-Liquid Extraction
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2023
Source: Laboratory of Dr. Jay Deiner — City University of New York Extraction is a crucial step in most chemical analyses. It entails removing the analyte from its sample matrix and passing it into the phase required for spectroscopic or chromatographic identification and quantification. When the sample is a solid and the required phase for analysis is a liquid, the process is called solid-liquid extraction. A simple and broadly applicable form of solid-liquid extraction entails combining the...
09:18
Purification of a Total Lipid Extract with Column Chromatography
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst The product of an organic solvent extraction, a total lipid extract (TLE), is often a complex mixture of hundreds, if not thousands, of different compounds. The researcher is often only interested in a handful of compounds. The compounds of interest may belong to one of several classes of compounds, such as alkanes, ketones, alcohols, or acids (Figure 1), and it may be useful to remove the compound classes to which it does...
08:50
Prostaglandin Extraction and Analysis in Caenorhabditis elegans
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Cited by 11 •
2013
In this paper, we describe an optimized procedure for extracting and analyzing prostaglandins and other eicosanoids from C. elegans using LC-MS/MS.
02:55
Extraction - Concepts
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2020
Extraction Extraction is a common technique used in organic chemistry to isolate a target compound. In the extraction process, a solute is transferred from one phase to another to separate it from unreacted starting materials or impurities. Extraction is also used to facilitate the isolation of a solute from a reaction solvent that is difficult to remove by evaporation, such as a solvent with a high boiling point. Generally, there are three types of extractions. First, in solid-liquid...
10:12
Extraction of Organochlorine Pesticides from Plastic Pellets and Plastic Type Analysis
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Cited by 10 •
2017
Microplastics act as vector of potentially toxic organic contaminants with unpredictable effects. This protocol describes an alternative methodology for assessing the levels of organochlorine pesticides adsorbed on plastic pellets and identifying the polymer chemical structure. The focus is on pressurized fluid extraction and attenuated total reflectance Fourier transform infrared spectroscopy.
FAQs
Selectivity comes from molecular polarity. Hexane preferentially dissolves hydrophobic molecules, including oils and fats, while most polar substances remain outside the solvent extract. This difference allows researchers to separate lipid-rich material from much of the surrounding biological matrix, making the resulting extract useful for estimating oil content or preparing nonpolar components for further chemical analysis.
The solvent can contain dissolved lipids while solid biological material remains behind. Filtration removes those solids, whereas phase separation can separate distinct portions of the extraction mixture. These cleanup steps reduce unwanted sample material in the liquid fraction before evaporation, improving the concentration and suitability of the recovered lipid material for analysis.
Evaporation removes the volatile solvent and leaves the dissolved, less volatile extract concentrated. This step changes the sample from a dilute hexane solution into a more manageable lipid-rich material for measuring oil content or examining fatty components. Because hexane is volatile and flammable, evaporation must occur under appropriate ventilation and safety controls.
A typical workflow begins by contacting the biological material with hexane so nonpolar compounds dissolve. The mixture then undergoes filtration or phase separation to remove solids or distinguish liquid portions. Finally, evaporation concentrates the extracted material. This sequence connects solvent selectivity with physical cleanup and concentration, producing a preparation suited to subsequent lipid analysis.
The essential materials are a biological sample containing potential lipids and hexane as the extracting solvent. The workflow also requires a way to remove solids or separate phases, followed by controlled evaporation. Since hexane is volatile and flammable, researchers must provide appropriate ventilation and safety controls throughout handling and solvent removal.
Biologists use this approach with plant tissues, animal samples, and microbial biomass when they need to isolate or quantify nonpolar material. The resulting extract can support oil-content measurements, characterization of fatty components, or preparation for chemical analysis. Its value lies in converting lipid content within diverse biological materials into a concentrated fraction for study.