Highseq 1000
Highseq 1000 - Related Videos
10:14
Retroviral Transduction of T-cell Receptors in Mouse T-cells
0 Views •
Cited by 21 •
2010
We present a protocol to produce antigen-specific mouse T-cells using retroviral...
08:20
Organotypic Hippocampal Slice Cultures
0 Views •
Cited by 53 •
2011
We describe a method to prepare organotypic hippocampal slices that can be easily adapted to other brain regions. Brain slices are laid on porous membranes and culture media is allowed to form an interface. This method preserves the gross architecture of the hippocampus for up to 2 weeks in culture.
09:13
Chromatographic Purification of Highly Active Yeast Ribosomes
0 Views •
Cited by 7 •
2011
Contamination of preparations of eukaryotic ribosomes purified by traditional methods by co-purifying nucleases and proteases negatively impacts on downstream biochemical and structural analyses. A rapid and simple chromatographic purification method is used to solve this problem using yeast ribosomes as a model system.
10:10
Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
0 Views •
Cited by 29 •
2009
The platelet adhesion cascade takes place in the presence of shear flow, a factor not accounted for in conventional (static) well-plate assays. This article reports on a platelet-aggregation assay utilizing a microfluidic well-plate format to emulate physiological shear flow conditions.
09:11
Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination
0 Views •
Cited by 32 •
2014
Latex agglutination testing is a simple, rapid and inexpensive method for serotyping Streptococcus pneumoniae, and has also been widely applied in diagnostic microbiology. This manuscript describes the in-house production of latex agglutination reagents, quality control procedures and the application of this technique to pneumococcal serotyping.
FAQs
Reversibly terminated nucleotides regulate incorporation during sequencing cycles. After a labeled nucleotide is incorporated into a growing DNA copy, imaging records its fluorescent signal before the next cycle proceeds. This cycle-resolved process links each detected color signal to a nucleotide position, allowing the instrument to build sequence information from repeated observations across clonally amplified fragments.
Clonal amplification creates groups of identical DNA fragments for analysis on the platform. Because the fragments share the same sequence, nucleotide incorporation across a group produces a corresponding fluorescent signal that imaging can record during each cycle. This arrangement connects the observed signal with the sequence of the amplified genetic material being examined.
Imaging captures the fluorescence emitted after labeled nucleotides are incorporated into DNA fragments. Each cycle therefore produces visual signal data associated with newly added bases. Software then interprets those signals and converts them into sequence data, connecting the chemical incorporation event with a digital representation that researchers can analyze for biological questions.
The workflow proceeds from genetic material to clonally amplified DNA fragments, followed by repeated incorporation and imaging cycles. Fluorescently labeled, reversibly terminated nucleotides provide the signals recorded during those cycles. Finally, software converts the collected signals into sequence data, which can be examined for genome content, expressed RNA, or genetic variation.
The choice depends on the biological material or question being investigated. Whole-genome sequencing supports examination of genetic material across the genome, whereas exome sequencing focuses on the genome’s exome. RNA sequencing provides information from RNA molecules. HiSeq 1000 systems support all three applications, enabling different views of genome content and gene-related biology.
The resulting datasets can support studies of gene expression, genome organization, disease-associated mutations, and other molecular features. Researchers interpret the sequence information in relation to the selected application, such as RNA sequencing for expression-related questions or genome and exome sequencing for genetic variation. These analyses connect nucleotide-level data with broader biological processes.