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Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
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Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

DOI:
10.3791/1644-v

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10:10 min

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October 27, 2009

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, , ,
1Fluxion Biosciences, Inc.

Chapters

  • 00:00Title
  • 00:57Introduction
  • 01:16Preparation of the Microfluidic Channels of a BioFlux Plate
  • 04:34Preparing the Blood with GPIIb/IIIa Inhibitory Antibody
  • 05:41Running the Flow Cell Experiment on the BF1000 Workstation
  • 08:19Platelet Adhesion and Aggregation Results
  • 08:58Conclusion

Summary

Automatic Translation

Automatic Translation

The platelet adhesion cascade takes place in the presence of shear flow, a factor not accounted for in conventional (static) well-plate assays. This article reports on a platelet-aggregation assay utilizing a microfluidic well-plate format to emulate physiological shear flow conditions.

Tags

Platelet AdhesionPlatelet AggregationMicrofluidic Flow CellsShear FlowWell-plate AssaysMicrofluidic Well-plate FormatPhysiological Shear FlowExtracellular ProteinsCollagen IVon Willebrand FactorActive PerfusionPneumatic PumpBufferPlatelet InteractionsFluorescent DyeFlow RatesPlatelet ActivationPlatelet Aggregation InhibitorsIC50 Dose Response Data

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