Ice Pack Method

The Ice Pack Method is a simple biological laboratory technique that uses a chilled pack to keep samples, reagents, or tissues at a low temperature during handling. Cooling removes heat from the surrounding container and slows temperature-dependent processes, including enzymatic reactions, cellular metabolism, and sample degradation, while limiting direct exposure to melting water. In biology, this method helps preserve sample integrity during collection, transport, dissection, or short-term experimental preparation. It offers an accessible alternative to specialized refrigeration for temporary cooling, although researchers must monitor temperature and prevent freezing when the material is sensitive to ice formation.

Ice Pack Method - Related Videos

Education

JoVE Science Education - Engineering

Single and Two-phase Flow in a Packed Bed Reactor

0 Views •

2023

Source: Kerry M. Dooley and Michael G. Benton, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA The goal of this experiment is to determine the magnitude of maldistribution in typical packed bed reactors in both single phase and two-phase (gas-liquid) flow and evaluate the effects of this maldistribution on pressure drop. The concepts of residence time distribution and dispersion are introduced through the use of tracers, and these concepts are related to physical...

Research

JoVE Journal - Engineering

Improved Polydimethylsiloxane (PDMS) Double Casting via Silicone Oil Treatment for Densely Packed Microstructure Replication

0 Views •

2025

This protocol presents an improved demolding procedure for the Polydimethylsiloxane (PDMS) double casting technique by introducing silicone oil as a non-adhesive barrier between PDMS layers. Unlike plasma or chemical surface modification methods, this approach requires no specialized or costly equipment, making it a more accessible and cost-effective alternative.

Lab-Scale Model to Evaluate Odor and Gas Concentrations Emitted by Deep Bedded Pack Manure

0 Views •

Cited by 4 •

2018

A protocol has been developed to measure gases, odors, and nutrient composition in lab-scaled bedded manure packs, which can be used to study ways to improve air quality in commercial cattle facilities using deep-bedded manure packs.

Optimization of An Air-Based Heat Management System for Dusty Particulate Matter-Covered Lithium-Ion Battery Packs

0 Views •

Cited by 2 •

2023

Here, we present the adaptive simulated annealing method (ASAM) to optimize an approximate quadratic response surface model (QRSM) corresponding to a dusty particulate matter-covered battery heat management system and fulfill the temperature drops back by adjusting the airflow velocities combination of system inlets.

Research

JoVE Journal - Biology
Free Sample

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

0 Views •

Cited by 13 •

2009

Lectin-conjugated POROS beads were employed for HPLC. Glycopeptide standards served as positive and negative controls. MARS-14 depleted, trypsin-digested human plasma was chromatographed and flow-through (FT) and bound fractions collected for ESI-LC-MS/MS analyses. Glycopeptides were enriched in the bound fraction as compared to FT.

View All Results

FAQs

Related Topics