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JoVE Journal
Biology

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A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
 

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

Article doi: 10.3791/1398
October 1st, 2009 Usage Statistics

Summary October 1st, 2009

Lectin-conjugated POROS beads were employed for HPLC. Glycopeptide standards served as positive and negative controls. MARS-14 depleted, trypsin-digested human plasma was chromatographed and flow-through (FT) and bound fractions collected for ESI-LC-MS/MS analyses. Glycopeptides were enriched in the bound fraction as compared to FT.

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