In Vitro Intestine
In Vitro Intestine - Related Videos
08:15
Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids
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Cited by 7 •
2019
This protocol describes how to mimic suckling-to-weaning transition in vitro using mouse late fetal intestinal organoids cultured for 30 days.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
10:52
Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model
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Cited by 4 •
2013
Performing cell culture assays for investigating bacterial adhesion and invasion under aerobic conditions is usually unrepresentative of the in vivo environment. A Vertical Diffusion Chamber model allows study of interactions of the human pathogen Campylobacter jejuni with intestinal epithelial cells under more in vivo-like conditions, resulting in enhanced bacterial invasion.
04:37
Intestinal Crypts Isolation: A Method to Isolate Whole Crypts from Small Intestine of Murine Model
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2023
In this video, we demonstrate a technique to isolate whole crypts from a mouse’s small intestine. The isolated whole crypts can be used to propagate long-lived and self-renewing 3D organoids in vitro.
09:51
Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids
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Cited by 13 •
2017
We present protocols for isolation of intestinal 3D structures from in vivo tissue and in vitro basement matrix embedded organoids, and detail different fixation and staining protocols optimized for immuno-labeling of microtubule, centrosomal, and junctional proteins as well as cell markers including the stem cell protein Lgr5.
FAQs
Cultured intestinal cells can be directed toward epithelial differentiation, allowing the model to represent specialized intestinal cell states rather than an undifferentiated cell population. As these cells organize, they can support nutrient transport and barrier formation. Studying these linked functions helps researchers connect cellular behavior with tissue-level processes relevant to digestion, absorption, and intestinal disease.
Stem-cell-derived organoids provide a three-dimensional approach for studying intestinal structure and function, whereas microfluidic gut-on-chip platforms use controlled device environments to reproduce selected tissue-level features. Both can support investigations of epithelial behavior and barrier function, but their formats offer different ways to examine intestinal biology, disease processes, and responses to experimental conditions.
Defined conditions allow researchers to control which cells, environmental factors, and biological interactions are present during an experiment. This control is especially important when examining nutrient transport, barrier formation, inflammation, infection, or microbiota interactions, because researchers can relate observed changes to selected experimental conditions rather than to uncontrolled variation within a whole organism.
Microbiota interactions can be examined by incorporating intestinal models with conditions that support contact or communication between cultured intestinal tissue and microorganisms. This approach enables controlled study of how microbial relationships relate to epithelial function, barrier properties, inflammation, or infection. It also helps separate specific biological interactions from the many variables present in an intact organism.
The principal components are cultured intestinal cells, stem-cell-derived organoids, or microfluidic gut-on-chip platforms. Researchers select among these formats according to the intestinal feature they need to examine, such as epithelial differentiation, nutrient transport, barrier formation, or microbiota interaction. Using these components under defined conditions creates a controlled experimental setting for linking cellular mechanisms with tissue-level function.
Researchers may use these models when they need controlled analysis of intestinal biology, disease, or treatment responses while reducing reliance on animal studies. The systems can support investigations of digestion, absorption, inflammation, infection, and drug toxicity. Because experimental conditions are defined, they also provide a platform for connecting specific cellular changes with broader intestinal outcomes.
These models can reveal changes in epithelial differentiation, nutrient transport, barrier formation, inflammation, infection, or drug toxicity. Such outcomes help researchers evaluate how intestinal tissue responds under controlled conditions and compare biological effects across experimental settings. The resulting information supports the development of more predictive therapies and can contribute to personalized medicine and regenerative biology.
In biology, these systems provide an intermediate level of investigation between isolated cellular mechanisms and whole-organism intestinal function. They allow researchers to study how cells organize into tissue-like systems and how that organization relates to disease or treatment responses. Their use also supports regenerative biology, personalized medicine, and efforts to develop therapies that better predict intestinal effects.