In Vivo Mouse Model
In Vivo Mouse Model - Related Videos
07:46
In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
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Cited by 39 •
2010
Orthotopic animal models of ovarian cancer replicate better human disease and therefore enhance our understanding of cancer progression and tumor response to therapy. A mouse model receives an intrabursal injection of luciferase-expressing ovarian tumor cells. Treatment is administered via oral gavage. Tumor growth is monitored by in vivo imaging system.
09:56
In Vivo Multimodal Imaging and Analysis of Mouse Laser-Induced Choroidal Neovascularization Model
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Cited by 7 •
2018
Here, we present the usefulness of longitudinal in vivo imaging in the follow-up of morphological changes of laser-induced choroidal neovascularization in mice.
07:03
Intrauterine Telemetry to Measure Mouse Contractile Pressure In Vivo
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Cited by 16 •
2015
This manuscript provides a protocol for implanting telemeters in the mouse for the purpose of measuring intrauterine pressures during pregnancy.
03:10
In Vivo Fluorescence Imaging to Localize Antibodies in a Mouse Tumor Xenograft Model
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2025
The video demonstrates a fluorescence imaging method for in vivo antibody localization in a mouse tumor xenograft model. It involves the injection of tumor cells in a mouse to generate solid xenografts beneath the skin. Subsequent injection of fluorescent antibodies confirms specific localization through enhanced fluorescence signals within the tumor xenograft.
12:31
In Vivo Modeling of the Morbid Human Genome using Danio rerio
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Cited by 66 •
2013
Here, we present a systematic approach for developing physiologically relevant, sensitive and specific in vivo assays for interpreting variation in human pathology. Transient genetic manipulation via microinjection of WT and mutant human mRNA and morpholino (MO) antisense oligonucleotides harness the tractability of the developing zebrafish embryo to rapidly assay pathogenic mutations, especially, but not exclusively, in the context of human developmental disorders.
FAQs
The selected condition determines which biological changes researchers can examine and how outcomes should be interpreted. A genetic condition can support studies of gene function, whereas a physiological or pathological condition can frame investigations of disease progression or intervention responses. Because these conditions exist within an intact organism, measurements can reflect coordinated effects across organs, tissues, and cell types.
An intact mouse preserves biological relationships among multiple organs, tissues, and cell types. These connections allow researchers to observe system-level effects rather than responses from one isolated biological component. This distinction matters when an intervention or disease process affects several parts of the organism at once, helping reveal interactions that cell-based or tissue-based studies cannot fully reproduce.
Findings from mice can reveal biological mechanisms, intervention responses, and useful hypotheses, but they do not automatically predict human outcomes. Mouse and human biology differ, so researchers must interpret results cautiously and consider the model’s relevance to the question being studied. This limitation is especially important when using animal findings to guide treatment development.
Researchers first introduce or observe a defined genetic, physiological, or pathological condition in living mice. They then measure selected outcomes across relevant organs, tissues, and cell types, with the measurements matched to the biological question. Depending on the study, the observations may address disease progression, gene function, drug activity, toxicity, or therapeutic efficacy.
This model is useful when the research question depends on interactions across the intact organism. It can support investigations of disease mechanisms, progression, gene function, drug activity, toxicity, and therapeutic efficacy. Researchers may choose it when isolated cells or tissues cannot capture the coordinated biological relationships needed to evaluate an intervention or understand a complex disease process.
Measurements can indicate whether an intervention produces a biological response, whether it shows toxicity, and whether it has therapeutic efficacy within the organism. The model can also help connect those outcomes with disease progression or underlying gene function. These results may guide new hypotheses and inform treatment development, while remaining subject to careful interpretation across species.