Gene Knockout Mice
Gene Knockout Mice - Related Videos
04:13
CRISPR Concatemer-Mediated Multiple Gene Knockout: A Technique to Simultaneously Knockout Multiple Genes by Non-Homologous End-Joining Pathway in Mouse Intestinal Cells
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2025
This video describes a gene knockout technique using a CRISPR-concatemer to simultaneously knock out multiple genes in cultured mouse intestinal organoid cells. This method is used to knock out a diseased gene and to elucidate the function of a gene and its paralogues.
02:29
In-vitro Mutagenesis (Gene Knockouts)
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2026
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes. The Process Genes can be randomly knocked out, or specific genes can be targeted. To knock out a particular gene, an engineered piece of DNA called a targeting vector is used to replace the...
11:53
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
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Cited by 17 •
2017
This protocol describes the steps for cloning multiple single guide RNAs into one guide RNA concatemer vector, which is of particular use in creating multi-gene knockouts using CRISPR/Cas9 technology. The generation of double knockouts in intestinal organoids is shown as a possible application of this method.
11:35
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
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Cited by 6 •
2017
Recent advances in the ability to genetically manipulate somatic cell lines hold great potential for basic and applied research. Here, we present two approaches for CRISPR/Cas9 generated knockout production and screening in mammalian cell lines, with and without the use of selectable markers.
03:12
CreER-LoxP System-Based Target Gene Inactivation: A Tamoxifen-Inducible Cre Recombinase System for Target Gene Knockout in a Mouse Model
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2025
This video describes a procedure for target gene knockout using a tamoxifen-inducible Cre-recombinase system following intraperitoneal injection of tamoxifen in a mouse model.
FAQs
Genetically matched controls help researchers attribute observed differences specifically to loss of the selected gene. Investigators compare the knockout animals with mice that share the relevant genetic background but retain the functional allele. This design supports clearer interpretation of changes in development, physiology, disease susceptibility, or treatment response, reducing the risk of confusing gene effects with unrelated genetic variation.
Conditional or tissue-specific knockouts help separate a gene’s developmental role from its function in an adult tissue. Removing the gene only in a selected tissue, or at a defined stage, can reveal effects that might be obscured when gene loss occurs throughout the animal’s development. This distinction is especially useful when interpreting physiological changes or disease-related phenotypes.
Both approaches produce an altered allele, but they begin with different experimental strategies. In one route, researchers disrupt the target gene in embryonic stem cells and then identify animals carrying the change. Genome-editing systems such as CRISPR-Cas9 provide another way to alter the selected sequence. Subsequent screening or breeding establishes animals suitable for comparison with controls.
The consequences of gene loss may appear in development, physiology, disease susceptibility, or responses to treatment. Researchers examine these outcomes to infer what biological processes depend on the selected gene. Comparing several types of phenotype can also connect a gene’s activity to normal function and to disease mechanisms, rather than limiting interpretation to a single observable trait.
A typical workflow begins by disrupting the selected gene in embryonic stem cells or with CRISPR-Cas9. Researchers then screen animals for the altered allele and may breed carriers to establish the experimental animals. Finally, they compare knockout mice with genetically matched controls and assess relevant developmental, physiological, disease-related, or treatment-response outcomes.
These models are useful when researchers need to connect loss of a particular gene with disease susceptibility or a measurable biological response. If the phenotype changes after treatment, the model can also help evaluate whether the gene represents a drug target. Such comparisons support investigation of disease mechanisms and the development of potential therapeutic strategies.
Knockout mice can show whether removing a gene changes disease-related traits or alters responses to treatment. Those results provide biological context for judging the gene’s relevance to a potential therapy. Conditional and tissue-specific designs add further value by indicating whether the therapeutic strategy should address gene function during development, in adulthood, or within a particular tissue.