Somatic Stem Cells
Somatic Stem Cells - Related Videos
07:06
Tissue Regeneration with Somatic Stem Cells
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2023
Somatic or adult stem cells, like embryonic stem cells, are capable of self-renewal but demonstrate a restricted differentiation potential. Nonetheless, these cells are crucial to homeostatic processes and play an important role in tissue repair. By studying and manipulating this cell population, scientist may be able to develop new regenerative therapies for injuries and diseases. This video first defines somatic stem cells, and then explores the role these cells play in tissue regeneration.
08:25
Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP
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Cited by 6 •
2012
A method to generate human induced pluripotent stem cells (iPSCs) via retrovirus-mediated ectopic expression of OCT4, SOX2, KLF4 and MYC is described. A practical way to identify human iPSC colonies based on GFP expression is also discussed.
09:43
Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
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Cited by 13 •
2014
Here, we present our established method to reprogram human somatic cells into transgene-free human iPSCs with Sendai virus, which shows consistent outcome and enhanced efficiency.
13:05
De Novo Generation of Somatic Stem Cells by YAP/TAZ
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Cited by 4 •
2018
Availability of somatic SCs is crucial for regenerative medicine, disease modeling and to gain insight into SC properties. Here we present experimental strategies to reprogram, in vitro, differentiated adult cells into their corresponding expandable tissue-specific stem/progenitor cells by the transient expression of the single transcriptional co-activator YAP.
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 8 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
FAQs
The surrounding stem-cell niche helps regulate the balance between self-renewal and production of progenitor cells. Signals from nearby tissue components influence whether the stem-cell population is maintained or directed toward generating specialized cells. This local control connects somatic stem-cell behavior with tissue homeostasis, allowing repair and maintenance to remain specific to the organ in which the cells reside.
Restricted developmental potential limits the range of specialized cells these stem cells can produce, but that limitation can improve tissue specificity. A population adapted to a particular organ may therefore be more relevant for repairing or modeling that tissue than a cell type with broader developmental potential. The same restriction also constrains possible therapeutic uses.
Self-renewal preserves the resident stem-cell population, while the production of progenitor cells supplies cells that can differentiate into specialized tissue types. Coordinating these outcomes helps an organ replace or repair cells without exhausting its stem-cell reserve. Disruption of this balance is therefore relevant to biological studies of tissue maintenance, aging, and disease.
Studying these tissue-associated populations reveals how stem-cell behavior differs across organs and biological contexts. Comparisons can address how each population contributes to tissue maintenance, regeneration, development, aging, or disease. They also help researchers evaluate which stem-cell types are most suitable for tissue-specific applications, including transplantation, organoid models, and cell-based therapies.
Somatic stem cells can provide tissue-relevant cellular material for organoid models, which are used to study organ biology in a controlled research setting. These models also support drug testing by providing a system connected to a particular tissue type. Their restricted developmental potential can help preserve tissue specificity when researchers examine responses or disease-related processes.
They are considered when a research or therapeutic strategy requires cells associated with a particular tissue and its repair processes. Their tissue residence and restricted developmental potential may support more targeted applications, while also limiting the range of replacement cells they can generate. Researchers therefore assess both their regenerative relevance and their developmental constraints when developing transplantation or cell-based approaches.