Inflammation Mediators
Inflammation Mediators - Related Videos
04:06
Using Fluorescence-Mediated Tomography to Assess Murine Intestinal Inflammation
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2025
This video demonstrates a technique for assessing intestinal macrophage infiltration in a murine model of colitis using fluorescence-mediated tomography (FMT). Upon inducing colitis in a mouse via dextran sodium sulfate (DSS), the animal is injected with antibodies that recognize the F4/80 glycoprotein marker on infiltrated macrophages in the colon. The mouse is then imaged in an FMT device where the fluorescence and reflectance signals are reconstructed into a three-dimensional image to...
07:05
Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation
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Cited by 2 •
2017
Target-specific probes represent an innovative tool for analyzing molecular mechanisms, such as protein expression in various types of disease (e.g., inflammation, infection, and tumorigenesis). In this study, we describe a quantitative three-dimensional tomographic assessment of intestinal macrophage infiltration in a murine model of colitis using F4/80-specific fluorescence-mediated tomography.
01:38
Inflammation
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2019
Overview In response to tissue injury and infection, mast cells initiate inflammation. Mast cells release chemicals that increase the permeability of adjacent blood capillaries and attract additional immune cells to the wound or site of infection. Neutrophils are phagocytic leukocytes that exit the bloodstream and engulf invading microbes. Blood clotting platelets seal the wound and fibers create a scaffold for wound healing. Macrophages engulf aging neutrophils to end the acute inflammatory...
06:48
The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events
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Cited by 1 •
2019
Here, we present a protocol to detect the presence of neutrophils in fixed/permeabilized histology sections and assess the activation state of live purified neutrophils. In particular, the MUB40 peptide binds lactoferrin present in neutrophil-specific and tertiary granules. Exposure of the granule contents through either permeabilization or neutrophil activation allows for the marking of neutrophils.
07:10
In vivo Imaging Method to Distinguish Acute and Chronic Inflammation
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Cited by 25 •
2013
We describe a non-invasive imaging method for distinguishing inflammatory stages. Systemic delivery of luminol reveals areas of acute inflammation dependent upon MPO activity in neutrophils. In contrast, injection of lucigenin allows for visualization of chronic inflammation dependent upon Phox activity in macrophages.
FAQs
The decisive step is receptor engagement on a target cell. A mediator released by an immune or damaged cell can influence nearby cells or travel farther, depending on where its receptor is present. Receptor signaling then changes vascular permeability, leukocyte recruitment, or inflammatory gene expression, coordinating responses across tissues.
Inflammatory signaling must be strong enough to address injury, infection, or tissue stress, yet controlled enough to resolve afterward. If mediator activity becomes excessive or persistent, the response can contribute to autoimmune disease, chronic inflammation, and tissue damage. This balance determines whether signaling supports protection and repair or produces harmful consequences.
Different mediator classes provide complementary signals rather than a single uniform instruction. Cytokines, chemokines, histamine, prostaglandins, and complement proteins are among the identified examples, and their combined activity can influence vessels, leukocyte movement, gene expression, and later resolution. This variety helps coordinate a response to changing tissue conditions.
After injury or tissue stress, mediator activity can help eliminate harmful stimuli while supporting tissue repair. The outcome depends on coordinated signaling and resolution rather than continued activation. Studying this balance gives biologists a way to examine how immune responses protect damaged tissue without allowing persistent activity to promote further tissue damage.
The same signaling network is relevant beyond immediate injury studies. Investigators examine inflammation mediators in infection and cancer alongside immune regulation, because their activity provides a biological context for understanding how inflammatory responses are organized and controlled. These studies also help connect mediator behavior with possible tissue damage or repair outcomes.
Because excessive or persistent mediator activity can contribute to autoimmune disease, chronic inflammation, and tissue damage, these molecules are important subjects in anti-inflammatory therapy research. Studying them focuses attention on how inflammatory signaling is regulated, helping distinguish harmful activity from responses that remain useful for eliminating harmful stimuli and supporting tissue repair.