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The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events
 

The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

Article DOI: 10.3791/58367-v
January 7th, 2019

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Summary January 7th, 2019

Here, we present a protocol to detect the presence of neutrophils in fixed/permeabilized histology sections and assess the activation state of live purified neutrophils. In particular, the MUB40 peptide binds lactoferrin present in neutrophil-specific and tertiary granules. Exposure of the granule contents through either permeabilization or neutrophil activation allows for the marking of neutrophils.

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