The MUB<sub>40</sub> Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

Mark C. Anderson; Louise Injarabian; Antonin Andre; Regis Tournebize; Benoit S. Marteyn

doi:10.3791/58367

Journal

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Biology

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The MUB₄₀ Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

The MUB<sub>40</sub> Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

JoVE Journal
Biology

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JoVE Journal Biology

The MUB₄₀ Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

6,101 Views

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06:48 min

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January 07, 2019

DOI:

10.3791/58367-v

DOI:

10.3791/58367-v

•

06:48 min

January 07, 2019

•

6086 Views

Mark C. Anderson^1,2, Louise Injarabian^1,2,3, Antonin Andre^1,2,3, Regis Tournebize^1,2,4, Benoit S. Marteyn^1,2

¹Unité de Pathogénie Microbienne Moléculaire,Institut Pasteur ²INSERM Unité 1202,Institut Pasteur ³Institut de Biochimie et Génétique Cellulaires,Université Bordeaux Segalen ⁴Unit of Technology and Service Photonic BioImaging,Institut Pasteur

Summary

Here, we present a protocol to detect the presence of neutrophils in fixed/permeabilized histology sections and assess the activation state of live purified neutrophils. In particular, the MUB₄₀ peptide binds lactoferrin present in neutrophil-specific and tertiary granules. Exposure of the granule contents through either permeabilization or neutrophil activation allows for the marking of neutrophils.