Here, we present a protocol to detect the presence of neutrophils in fixed/permeabilized histology sections and assess the activation state of live purified neutrophils. In particular, the MUB40 peptide binds lactoferrin present in neutrophil-specific and tertiary granules. Exposure of the granule contents through either permeabilization or neutrophil activation allows for the marking of neutrophils.
Anderson, M. C., Injarabian, L., Andre, A., Tournebize, R., Marteyn, B. S. The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events. J. Vis. Exp. (143), e58367, doi:10.3791/58367 (2019).