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The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events
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The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

DOI:
10.3791/58367-v

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06:48 min

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January 07, 2019

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, , , ,
1Unité de Pathogénie Microbienne Moléculaire,Institut Pasteur, 2INSERM Unité 1202,Institut Pasteur, 3Institut de Biochimie et Génétique Cellulaires,Université Bordeaux Segalen, 4Unit of Technology and Service Photonic BioImaging,Institut Pasteur

Chapters

  • 00:04Title
  • 00:34Visualization of Neutrophil Activation with Reverse-Inverso-MUB40-Cy5 Peptide
  • 05:16Results: RI-MUB40 Staining Increases Over Time as More Neutrophils Become Activated
  • 06:03Conclusion

Summary

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Here, we present a protocol to detect the presence of neutrophils in fixed/permeabilized histology sections and assess the activation state of live purified neutrophils. In particular, the MUB40 peptide binds lactoferrin present in neutrophil-specific and tertiary granules. Exposure of the granule contents through either permeabilization or neutrophil activation allows for the marking of neutrophils.

Tags

Keywords: MUB40 PeptideNeutrophil MarkerLactoferrinNeutrophil DetectionInflammatory DiseasesAnoxic CabinetCell SeparationPercoll GradientNeutrophil IsolationWashing Buffer

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