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The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events
The MUB<sub>40</sub> Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events
JoVE Journal
Biology
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JoVE Journal Biology
The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

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06:48 min

January 07, 2019

DOI:

06:48 min
January 07, 2019

6086 Views
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Summary

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Here, we present a protocol to detect the presence of neutrophils in fixed/permeabilized histology sections and assess the activation state of live purified neutrophils. In particular, the MUB40 peptide binds lactoferrin present in neutrophil-specific and tertiary granules. Exposure of the granule contents through either permeabilization or neutrophil activation allows for the marking of neutrophils.

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