Intraluminal Ph
Intraluminal Ph - Related Videos
06:20
Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure
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Cited by 22 •
2011
This is a method to visualise leukocyte adhesion to the endothelium in harvested pressurised vessels. The technique enables studying vascular adhesion under shear flow with differing intraluminal pressures up to 200 mmHg thus mimic-ing the pathophysiological conditions of high blood pressure.
05:53
Intraluminal Drug Delivery to the Mouse Arteriovenous Fistula Endothelium
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Cited by 5 •
2016
After puncturing the aorta through the inferior vena cava (IVC) to create an aorto-caval fistula in the mouse, solution containing a drug is infused into the IVC via the same needle, followed by incubation. This method enables more robust drug delivery to the venous endothelium compared to the external route.
15:01
Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy
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Cited by 21 •
2011
We describe a protocol of brightfield intravital microscopy for measuring dynamic neutrophil-endothelial cell interactions during neutrophil recruitment in response to the source of a neutrophil chemoattractant in vivo. Neutrophil intraluminal crawling, transendothelial migration and chemotaxis in mouse cremaster muscle tissue are visualized with time-lapsed video photography and tracked with ImageJ.
09:40
Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining
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Cited by 97 •
2012
The intraluminal middle cerebral occlusion model in mice is herein presented. The extent of cerebral infarct is evaluated by a neurologic score and cresyl violet staining, an alternative staining to TTC, offering the great advantage to test in parallel many interest markers.
06:20
In Vitro Intraluminal Gel Infusion: An Advanced Approach for Microscopic Analysis of Human Resistance Arteries
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2025
Here, we present a protocol for preserving human arterial architecture by infusing tissue-stabilizing gel into the vessel lumen before sectioning for molecular or histopathological analysis.
FAQs
Intraluminal pH reflects the balance between hydrogen ion production, secretion, and removal within a lumen. Epithelial cells can transport chemical species across the lining, while glandular secretions add acids, bases, or buffering substances. Dilution by incoming contents further changes the local value, so pH can vary substantially between regions of the same organ.
Different gastrointestinal regions receive distinct secretions and experience different levels of dilution, buffering, and epithelial transport. These conditions create pH gradients rather than a uniform chemical environment. The resulting regional differences influence where digestive processes occur, how effectively enzymes function, and which nutrients or other substances can be absorbed.
Resident microorganisms contribute to local acidity or alkalinity through their metabolism of luminal contents. Their activity can therefore modify the chemical environment alongside host secretions and epithelial transport. Because microbial composition is itself influenced by these conditions, intraluminal pH and the resident microbial community can affect one another within the gastrointestinal tract.
A drug designed to respond to acidity or alkalinity may release its contents differently as it encounters changing luminal conditions. Regional pH gradients can therefore influence where release occurs and how a formulation behaves during transit. Measuring these conditions helps researchers evaluate delivery strategies intended to protect a compound or target its release to a particular location.
Researchers can characterize luminal conditions with pH probes, ingestible capsules, or other analytical methods. The selected approach provides measurements from within the hollow space rather than relying only on surrounding tissue. Repeated or region-specific observations can reveal spatial or temporal changes, supporting analysis of gastrointestinal physiology, disease mechanisms, and pH-dependent delivery systems.
Patterns in intraluminal pH provide functional information about the interaction of secretions, epithelial transport, dilution, buffering, digestion, absorption, and microbial activity. Researchers can compare these patterns across locations or biological conditions to investigate altered physiology and disease mechanisms. The same measurements also help connect local chemistry with enzyme activity and microbial composition.