Islet Equivalents Ieq
Islet Equivalents Ieq - Related Videos
07:21
Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells
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2023
Pancreatic islets, also called the Islets of Langerhans, are a cluster of endocrine cells which produces hormones for glucose regulation and other important biological functions. This protocol explains a method for isolating pancreatic islets by first perfusing collagenase via Ampulla of Vater to digest the exocrine pancreatic tissue and a simplified density gradient centrifugation to purify the islets from mice.
03:33
Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging
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2023
This video describes the protocol for grafting human pancreatic islets into the anterior chamber of the mouse’s eye to monitor revascularization of human islet grafts in vivo. It also helps to understand the contribution of recipient versus donor cells in promoting the encapsulation and vascularization of the graft.
03:48
Murine Model of Islet Transplantation: A Surgical Method to Infuse Pancreatic Islets in Portal Vein and Prevention of Postoperative Bleeding
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2025
In this video, we describe a procedure to infuse pancreatic islet cells into the hepatic portal vein, followed by application of gel foam to successfully prevent bleeding. Postoperative bleeding is a common cause of high mortality during islet transplantation.
07:04
Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
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Cited by 21 •
2017
We describe a protocol herein for isolating intact islets from neonatal mice. Pancreata were partially digested with collagenase, followed by washing and hand picking. 20 - 80 islet clusters can be obtained per pancreas from newly born mice, which are suitable for several islet studies.
06:09
Detection of Human Islet Autoantibodies Using an Electrochemiluminescence Assay
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2025
This video showcases the electrochemiluminescence detection of islet autoantibodies in human serum. The antibodies in the serum bridge the ruthenium-conjugated antigen to the biotinylated capture antigen, forming complexes that are detected by electrochemiluminescence measurements.
FAQs
The reference size provides a common scale for expressing pancreatic islet mass. Because isolated preparations contain islets of different diameters, converting their estimated combined volume into this standardized unit makes yields comparable between samples. This is particularly useful when laboratories evaluate preparations that differ in size distribution but need a consistent basis for biological comparison.
Size distribution is essential because the estimate depends on both how many islets are present and how large they are. Two preparations with the same islet count can therefore produce different IEq values if their islets have different volumes. Measuring the distribution prevents a simple particle count from representing the total islet mass inaccurately.
A count records the number of islets, whereas IEq incorporates the estimated volume represented by those islets. This distinction matters because isolated preparations are not uniform in size. IEq therefore gives researchers a more useful standardized measure for comparing total islet mass, while the raw count alone cannot account for size-related differences between preparations.
Researchers first assess the number of islets and the distribution of their sizes within the isolated preparation. They then estimate the combined islet volume and express that volume using the standardized IEq reference. The resulting value can be considered alongside preparation purity, viability, and insulin-secretory performance when evaluating the quality of the isolation.
No. IEq standardizes estimated islet mass, but it does not independently establish viability or insulin-secretory performance. Those properties must be assessed as additional characteristics of the preparation. Using IEq together with these measures helps distinguish how much islet material is present from how well that material may function in biological or transplantation-related studies.
IEq is used to compare pancreatic islet isolation yields, help optimize transplantation doses, and assess engineered or donor-derived islet products. Its value comes from giving these applications a common measure of islet mass despite size differences. Researchers can then interpret mass together with purity, viability, and insulin secretion when judging preparation quality and experimental suitability.