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JoVE Journal
Developmental Biology

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Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
 

Effective Isolation of Functional Islets from Neonatal Mouse Pancreas

Article doi: 10.3791/55160
January 6th, 2017

Summary January 6th, 2017

We describe a protocol herein for isolating intact islets from neonatal mice. Pancreata were partially digested with collagenase, followed by washing and hand picking. 20 - 80 islet clusters can be obtained per pancreas from newly born mice, which are suitable for several islet studies.

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