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Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
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Developmental Biology
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JoVE Journal Developmental Biology
Effective Isolation of Functional Islets from Neonatal Mouse Pancreas

Effective Isolation of Functional Islets from Neonatal Mouse Pancreas

DOI:
10.3791/55160-v

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07:04 min

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January 06, 2017

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1Department of Cell and Development Biology,Vanderbilt University Medical School

Chapters

  • 00:05Title
  • 00:39Pancreas Isolation and Digestion
  • 02:14Lysate Washing and Islet Isolation
  • 04:42Results: Representative Islet Isolation Analyses
  • 05:39Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol herein for isolating intact islets from neonatal mice. Pancreata were partially digested with collagenase, followed by washing and hand picking. 20 - 80 islet clusters can be obtained per pancreas from newly born mice, which are suitable for several islet studies.

Tags

Keywords: Neonatal Mouse PancreasIslet IsolationCollagenase DigestionHBSSRPMI 1640PolysucroseSodium DiatrazoateInsulin SecretionGene ExpressionParacell SurvivalFunctional Maintenance

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