Islet Yield Assessment
Islet Yield Assessment - Related Videos
07:04
Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
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Cited by 19 •
2017
We describe a protocol herein for isolating intact islets from neonatal mice. Pancreata were partially digested with collagenase, followed by washing and hand picking. 20 - 80 islet clusters can be obtained per pancreas from newly born mice, which are suitable for several islet studies.
09:31
Assessing Replication and Beta Cell Function in Adenovirally-transduced Isolated Rodent Islets
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Cited by 16 •
2012
This protocol allows one to identify factors that modulate functional beta cell mass to find potential therapeutic targets for the treatment of diabetes. The protocol consists of a streamlined method to assess islet replication and beta cell function in isolated rat islets following manipulation of gene expression with adenoviruses.
01:31
ATP Yield
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2019
Cellular respiration produces 30 - 32 ATP per glucose molecule. Although most of the ATP results from oxidative phosphorylation and the electron transport chain (ETC), 4 ATP are gained beforehand (2 from glycolysis and 2 from the citric acid cycle). The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
03:33
Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging
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2023
This video describes the protocol for grafting human pancreatic islets into the anterior chamber of the mouse’s eye to monitor revascularization of human islet grafts in vivo. It also helps to understand the contribution of recipient versus donor cells in promoting the encapsulation and vascularization of the graft.
07:21
Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells
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2023
Pancreatic islets, also called the Islets of Langerhans, are a cluster of endocrine cells which produces hormones for glucose regulation and other important biological functions. This protocol explains a method for isolating pancreatic islets by first perfusing collagenase via Ampulla of Vater to digest the exocrine pancreatic tissue and a simplified density gradient centrifugation to purify the islets from mice.
FAQs
Islet equivalents normalize measurements to a standard islet size, making preparations with differently sized clusters easier to compare. A simple cluster count could give disproportionate weight to many small or fewer large islets. Using islet equivalents therefore provides a more informative estimate of recovered islet material when researchers evaluate preparation success or compare isolation protocols.
Yield indicates how much islet material was recovered, but it does not fully describe preparation quality. Purity indicates the contribution of islet material within the preparation, while viability addresses the condition of the recovered cells. Considering these measures together helps researchers distinguish a large recovery from a preparation suitable for transplantation or experimental study.
Isolation conditions affect how much insulin-producing cell cluster material is recovered, so yield data can reveal differences between tissue-processing approaches. Interpreting the measurements alongside the protocol helps researchers determine whether changes in processing correspond to improved or reduced recovery. This comparison also supports reproducibility by linking observed outcomes to the conditions used during isolation.
A basic workflow combines microscopic examination with quantitative estimation of islet equivalents. Researchers inspect the recovered material, count islet structures, and use the observations to estimate the amount represented by a standardized islet size. Purity and viability may then be added as complementary measurements, producing a broader assessment of both recovery and preparation quality.
Researchers use yield measurements when they need to compare how effectively different isolation protocols recover pancreatic islets. Recording islet equivalents provides a common quantitative basis, while purity and viability add context about the resulting preparation. Monitoring these values across tissue-processing experiments can identify protocol differences and improve consistency between preparations and studies.
The measurements help researchers select preparations for transplantation or experimental studies by documenting recovered amount alongside quality-related characteristics. In studies of glucose regulation and diabetes, these data clarify how many insulin-producing cell clusters were obtained and whether the preparation meets the needs of the experiment. Consistent assessment also strengthens comparisons among biological preparations.