Kidney Slice Imaging
Kidney Slice Imaging - Related Videos
02:43
Fluorescence Imaging of Bacterial Colonization in Mouse Kidney Tissue
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2026
Source: Behera, R. K., et al. A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues. J. Vis. Exp. (2019)This video demonstrates a method for preparing infected mouse kidney tissue, staining DNA, and detecting bacterial gene expression using dual fluorescent reporters. Red marks all bacteria, and green highlights infection-responsive cells.
03:43
A Whole-Mount Organ Staining Technique for Three-Dimensional Imaging of the Kidney
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2025
This video demonstrates an assay for the whole-mount staining of murine kidneys using a tissue-clearing method called CUBIC (clear, unobstructed brain/body imaging cocktails and computational analysis) and immunofluorescent staining. The kidney is subjected to tissue clearing, wherein the kidney is delipidated, decolorized, and treated with a refractive index matching solution to make the tissue transparent for optimum three-dimensional imaging. The tissue is stained with fluorescently labeled...
08:19
The Mouse Isolated Perfused Kidney Technique
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Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
03:14
Vibratome Sectioning of Whole Murine Kidney: A Technique to Generate Thin Sections of Murine Kidney
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2023
In this video, we demonstrate the vibratome sectioning of a whole mouse kidney to obtain thin slices. These sliced tissue sections can be stored at low temperatures until further downstream analysis.
09:10
Preparation of Acute Subventricular Zone Slices for Calcium Imaging
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Cited by 2 •
2012
A method to load subventricular zone (SVZ) cells with calcium indicator dyes for recording calcium activity is described. The postnatal SVZ contains tightly packed cells including neural progenitor cells and neuroblasts. Rather than using bath loading we injected the dye by pressure inside the tissue allowing better dye diffusion.
FAQs
The choice determines whether imaging can focus on tissue that remains viable or on prepared tissue sections for structural examination. Viable slices support observation of activity in an intact tissue context, whereas fixed slices support examination of preserved morphology. This distinction helps align the preparation with questions about renal function, cellular structure, or injury-related changes.
Bright-field, fluorescence, and other optical methods can be selected according to the structural or activity-related feature under study. Their use allows investigators to examine nephron organization, cellular morphology, and spatial relationships within the same tissue architecture. This makes the imaging readout useful for connecting visible cellular features with larger patterns of renal organization.
Architecture keeps nephrons, tubules, vessels, and neighboring cells in their spatial relationships. That context is important because filtration and tubular transport depend on organized renal structures, while vascular organization helps place those processes within the tissue. Compared with isolated-cell studies, slice imaging therefore provides more spatial relevance without requiring the scope of a whole-animal experiment.
Researchers prepare thin sections from either viable or fixed kidney tissue, then examine them with bright-field, fluorescence, or another optical imaging method. The workflow links physical sectioning with microscopy so that tissue architecture remains available during observation. The resulting images can be used to assess structure and activity at the level of nephrons and individual cellular features.
It can reveal nephron organization, cellular morphology, and spatial relationships within kidney tissue. Depending on the research question, investigators can use those observations to study filtration, tubular transport, or vascular organization. Because these features are viewed together, the method can connect cellular appearance with tissue-level renal physiology rather than reporting morphology without its anatomical setting.
Kidney slice imaging is useful when researchers need to examine how renal tissue responds to injury or pharmacological treatment while retaining tissue-level organization. Imaging can show changes in cellular morphology, nephron structure, or spatial relationships associated with those conditions. It therefore supports investigation of disease mechanisms and therapeutic effects with greater biological relevance than isolated-cell observations alone.