Leaf Disc Homogenization
Leaf Disc Homogenization - Related Videos
01:23
Test for Homogeneity
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2023
The goodness–of–fit test can be used to decide whether a population fits a given distribution, but it will not suffice to decide whether two populations follow the same unknown distribution. A different test, called the test for homogeneity, can be used to conclude whether two populations have the same distribution. To calculate the test statistic for a test for homogeneity, follow the same procedure as with the test of independence. The hypotheses for the test for homogeneity can be stated as...
11:09
Relating Stomatal Conductance to Leaf Functional Traits
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Cited by 39 •
2015
Unraveling how physiology and morphology are linked allows a deeper understanding of mechanistic functioning of plant leaves. We present both a procedure to derive parameters of stomatal regulation from stomatal conductance measurements and correlations with traditional functional leaf traits.
10:49
Measuring Rates of Herbicide Metabolism in Dicot Weeds with an Excised Leaf Assay
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Cited by 14 •
2015
This manuscript describes how herbicide metabolism rates can be effectively quantified with excised leaves from a dicot weed, thereby reducing variability and removing any possible confounding effects of herbicide uptake or translocation typically observed in whole-plant assays.
08:14
LeafJ: An ImageJ Plugin for Semi-automated Leaf Shape Measurement
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Cited by 93 •
2013
Demonstration of key methods for high throughput leaf measurements. These methods can be used to accelerate leaf phenotyping when studying many plant mutants or otherwise screening plants by leaf phenotype.
14:33
Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species
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Cited by 13 •
2012
The generation, purification and cell invasion of intracellular, cytoplasmic full length DISC1 protein aggresomes from cell cultures and of a labeled, multimeric recombinant DISC1 protein fragment in E. coli are described. Cell invasiveness is shown for recipient cells in cell culture and for neurons in vivo after stereotactical brain inoculation.
FAQs
Uniform disruption helps distribute cellular material throughout the tissue homogenate rather than leaving some regions relatively intact. This produces a more representative sample for subsequent measurements, so detected differences are more likely to reflect biological variation in the leaf tissue rather than uneven release of its contents. Consistency is especially relevant when comparing plant responses or cellular composition.
The extraction buffer receives cellular contents as cutting, grinding, or mixing breaks leaf cell walls and membranes. Dispersing released material throughout this liquid phase helps generate a workable homogenate for isolating or measuring pigments, proteins, nucleic acids, enzymes, and metabolites. Its central role is therefore connected to sample recovery and preparation for downstream biochemical or molecular analysis.
Mechanical force physically breaks the structures that keep cellular components enclosed within leaf tissue. Once cell walls and membranes are disrupted, pigments, proteins, nucleic acids, enzymes, and other metabolites can move into the surrounding buffer. The resulting dispersion makes these components accessible for later isolation or measurement, linking the physical preparation step to the analytical goals of the experiment.
A basic workflow begins with circular pieces of leaf tissue, followed by mechanical disruption through cutting, grinding, or mixing. The disrupted material is dispersed in an extraction buffer to produce a uniform homogenate. That preparation can then be used for a selected biological assay, depending on whether the study focuses on pigments, proteins, nucleic acids, enzymes, or metabolites.
This preparation can support the isolation or measurement of several classes of leaf components, including pigments, proteins, nucleic acids, enzymes, and other metabolites. Because the homogenate provides dispersed cellular material, researchers can adapt it for downstream biochemical or molecular assays. The specific measurement depends on the component of interest and the analytical procedure that follows sample preparation.
Biologists can use these samples when they need to compare plant responses or investigate the cellular composition of leaf tissue. A representative homogenate provides material for examining molecular and biochemical components in a common sample format. This makes the technique useful as an upstream preparation step in studies that connect tissue-level observations with pigment, protein, nucleic-acid, enzyme, or metabolite measurements.