Leaf Epidermis

The leaf epidermis is the outer protective cell layer of a plant leaf, separating internal tissues from the environment while supporting photosynthesis and water balance. Its tightly arranged cells often secrete a waxy cuticle that limits water loss, while specialized guard cells regulate stomatal openings to control carbon dioxide uptake, oxygen release, and transpiration. Epidermal hairs, or trichomes, can further reduce herbivory, reflect excess light, and modify the leaf’s surface climate. Studying leaf epidermis helps biologists understand plant adaptation, gas exchange, drought responses, and interactions with pathogens, with applications in ecology, agriculture, and plant physiology.

Leaf Epidermis - Related Videos

Education

JoVE Core - Anatomy and Physiology

Cells of the Epidermis

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2023

The epidermis is made of four or five layers of epithelial cells, depending on its location in the body. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum. The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...

Research

JoVE EoE - Immunopathology

Establishment of Rice Plant Leaf Infection through Fungal Inoculation via Wounding

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2025

This video demonstrates the establishment of rice plant leaf infection through wounding inoculation with Magnaporthe grisea mycelium. The fungal spore-derived germ tube penetrates the epidermal layer, resulting in tissue infection and lesions on the leaves. This method aids pathotype screening and improves plant resistance understanding for molecular breeding.

Layers of the Epidermis

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2025

The epidermis, the outermost layer of the skin, is composed of several distinct layers. From deep to superficial, the layers of the epidermis are as follows: Stratum Basale Stratum basale, also known as the stratum germinativum, is the deepest layer of the epidermis. It is composed of a single layer of actively dividing cells called basal cells or basal keratinocytes. These cells constantly undergo cell division to replenish the upper layers of the epidermis. Additionally, melanocytes, which...

Live Imaging of Cell Extrusion from the Epidermis of Developing Zebrafish

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Cited by 20 •

2011

Dying cells are extruded from epithelial tissues by concerted contraction of neighboring cells without disrupting barrier function. The optical clarity of developing zebrafish provides an excellent system to visualize extrusion in living epithelia. Here we describe methods to induce and image extrusion in the larval zebrafish epidermis at cellular resolution.

Research

JoVE Journal - Biology
Free Sample

Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies

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Cited by 2 •

2008

We describe a reliable method for immunolocalization within the epidermis modified for both frozen and paraffin sections that we use very routinely in our laboratory.

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