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Chapters
- 00:05Title
- 01:59Injection of mRNA Encoding the Fluorescently-tagged Actin Binding Protein
- 03:47Induction of Cell Extrusion in Zebrafish Larvae Using G418
- 04:47Mounting Zebrafish Larvae for Imaging
- 05:34Live Imaging of Actin Dynamics and Individual Epithelial Cell Behaviors during Cell Extrusion Using a Spinning Disc Confocal Microscope
- 08:22Representative Epidermal Images
- 08:55Conclusion
Dying cells are extruded from epithelial tissues by concerted contraction of neighboring cells without disrupting barrier function. The optical clarity of developing zebrafish provides an excellent system to visualize extrusion in living epithelia. Here we describe methods to induce and image extrusion in the larval zebrafish epidermis at cellular resolution.
Tags
Live ImagingCell ExtrusionEpidermisDeveloping ZebrafishDamaged CellsBarrier FunctionDying CellsActin And MyosinEpithelial SheetCell Extrusion ProcessOptical ClarityLarval Zebrafish EpidermisFluorescent Protein Labeled ProbeF-actinTransgenic Zebrafish EmbryosGreen Fluorescent ProteinApoptosisG418Time-lapse ImagingSpinning Disc Confocal MicroscopeActin DynamicsEpithelial Cell BehaviorsApoptotic Cell Elimination
