Lentiviral Particles
Lentiviral Particles - Related Videos
03:31
Laser-Assisted Lentiviral Gene Delivery: A Technique to Permeabilize Mouse Fertilized Eggs to Facilitate Lentiviral Gene Delivery
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2025
In this video, we demonstrate the laser-assisted permeation of the protective layer of zona pellucida in mouse fertilized eggs for facilitating lentiviral gene delivery. Lentivirus enables the generation of transgenic animals with a gene of interest stably integrated into their genome.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
03:07
Lentiviral Vector-Based Transduction of Rat Fertilized Embryos
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2026
Source: Koza, P., et al. Generation of Transgenic Rats using a Lentiviral Vector Approach. J. Vis. Exp. (2020)This video demonstrates microinjection-assisted delivery of lentiviral vectors into fertilized rat embryos to achieve stable transgene integration for the generation of transgenic rats.
09:53
Specific Marking of HIV-1 Positive Cells using a Rev-dependent Lentiviral Vector Expressing the Green Fluorescent Protein
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2010
We have developed a lentiviral vector that possesses, in addition to the Tat-responsive LTR, the Rev-response element (RRE) that can regulate reporter gene expression in an HIV-1 Tat- and Rev-dependent fashion. The vector permits the specific detection of replicating HIV in living cells via the expression of GFP.
16:04
A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
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Cited by 6 •
2013
Here, we use retroviral transduction and concatemeric transfection to create a cell line that can express the components of a lentiviral vector (LV) in the absence of tetracycline. This LV encodes GFP and is pseudotyped with a glycoprotein, SVGmu, which is specific for a receptor on dendritic cells.
FAQs
After a particle enters a mammalian cell, it releases its RNA genome into the cell. Reverse transcription converts that RNA into DNA, which is then transported to the nucleus. The delivered DNA can integrate into the host genome, allowing the inserted sequence to remain in the cell and support expression over time.
Integration can provide stable retention of a delivered sequence rather than relying only on genetic material that remains temporarily available inside the cell. This feature is especially useful when researchers want sustained expression or need to generate genetically modified cell lines. It also explains why vector design and control of components require careful consideration.
These vectors are valuable when target cells divide slowly or prove difficult to transfect using other approaches. Their ability to deliver genetic material and support stable expression broadens the range of mammalian cell types suitable for molecular and cell biology experiments. Researchers can therefore study cellular functions in systems that may be challenging to modify.
Engineered vector components determine how the delivery system performs and must be controlled carefully during experimental design and use. Component selection is relevant to the genetic material being delivered, the intended expression outcome, and biosafety considerations. Maintaining appropriate control helps researchers obtain interpretable gene-transfer results while managing risks associated with the vector system.
A high-level workflow involves selecting a vector carrying the sequence of interest, introducing the particles to mammalian target cells, and allowing entry, reverse transcription, nuclear transport, and possible genomic integration to occur. Researchers then use the modified cells for expression studies, functional experiments, or cell-line development, while applying appropriate biosafety practices throughout.
In functional genomics, researchers use these particles to deliver sequences that support investigations of gene function in mammalian cells. In disease modeling, the same delivery capability helps establish genetically modified cellular systems for studying disease-related biology. Their value comes from combining genetic delivery with the potential for stable expression in relevant cell models.
Depending on the experimental goal, lentiviral particles can enable gene transfer, sustained expression of a delivered sequence, and production of genetically modified cell lines. These outcomes support studies of cellular mechanisms, functional genomics, and disease models. Because integration can alter the host genome, researchers must pair outcome assessment with careful vector control and suitable biosafety procedures.