Lesion Extension
Lesion Extension - Related Videos
28:40
Proboscis Extension Response (PER) Assay in Drosophila
0 Views •
Cited by 120 •
2007
Proboscis extension response or PER is a taste behavior assay that has been used in flies as well as in honeybees. When the proboscis makes contact with an attractive substance, the fly extends its proboscis to consume the substance. Solutions of various sugars are very attractive to the fly.
03:32
Using a Macrophage Conditioned Medium to Promote Neuron Extensions
0 Views •
2025
This video demonstrates an assay for promoting the growth of neuron projections known as neurite outgrowth using the macrophage-conditioned medium. Co-culture neurons and macrophages with cAMP and harvest the macrophage-derived conditioned medium. Growing the neurons with the conditioned medium promotes neurite outgrowths. These outgrowths are fluorescently labeled and visualized using a fluorescence microscope.
09:22
Sectioning Mammary Gland Whole Mounts for Lesion Identification
0 Views •
Cited by 13 •
2017
We developed a method to successfully remove, process, section, and stain, for histopathological evaluation, mammary tissue that had originally been fixed on slides as whole mounts. This method may promote the collection and evaluation of mammary gland whole mounts in reproductive and developmental test guideline studies.
08:16
Comparative Lesions Analysis Through a Targeted Sequencing Approach
0 Views •
2019
This article describes a method to identify clonal and subclonal alterations among different specimens from a given patient. Although the experiments described here focus on a specific tumor type, the approach is broadly applicable to other solid tumors.
07:03
Initiating Rapid Neurite Extension and Formation Functional Neuronal Connections
0 Views •
2025
This video demonstrates a technique for the rapid initiation and extension of a neuron and creation of new connections with a target neuron using a microfluidic device and micromanipulation. This could be used for therapies that aim to reconnect neuronal circuits after trauma or in neurodegenerative diseases.
FAQs
Lesion extension reflects the continued influence of cellular injury, inflammation, or pathogen activity beyond the initially affected area. As these damaging influences affect adjacent tissue, the measurable boundary can advance. Tracking that advance helps researchers relate visible tissue change to ongoing biological processes and distinguish limited damage from progressively expanding injury.
Measurements collected at defined time points show how tissue damage changes rather than providing only a single snapshot. Comparing lesion area or diameter across those intervals reveals whether the boundary remains limited or continues to advance. Consistent timing also supports comparisons among organisms, experimental conditions, and treatment outcomes.
The amount and pattern of lesion growth provide a measurable indicator of how an organism responds to damaging influences. Smaller or more limited changes can be examined alongside larger extensions when characterizing host responses and evaluating disease resistance. This approach connects tissue-level observations with differences among organisms or experimental conditions.
A basic workflow records the lesion at standardized time points, uses imaging to document its visible boundary, and quantifies the affected area or diameter. Researchers then compare those measurements over time and across experimental groups. Standardization is important because consistent imaging and analysis make changes in tissue damage more reliable to interpret.
Treatment effects can be assessed by comparing lesion measurements from treated and untreated or otherwise different experimental conditions. Changes in area or diameter over the observation period indicate whether tissue damage progresses differently between groups. This provides a quantitative outcome for determining how an experimental treatment alters lesion development.
A standardized measure of lesion growth creates a common basis for comparing organisms, conditions, and outcomes. Researchers can examine differences in lesion area or diameter while using consistent imaging and quantitative analysis. In biology, these comparisons help characterize host responses, evaluate disease resistance, and relate experimental conditions to the severity or progression of tissue damage.