Live Optical Microscopy
Live Optical Microscopy - Related Videos
08:31
Three-dimensional Optical-resolution Photoacoustic Microscopy
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Cited by 27 •
2011
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging schemes.
15:39
Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm
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Cited by 2 •
2017
We demonstrate the use of various microscopy methods that are useful in observing the calcification of a tubeworm, Hydroides elegans, as well as locating and characterizing the first calcified material. Live microscopy and electron microscopy are used together to provide functional and material information that are important in studying biomineralization.
09:20
Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy
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Cited by 17 •
2010
This paper aims to instruct the reader in the operation of an integrated atomic force-optical imaging microscope for mechanical stimulation of live cells in culture. A step-by-step protocol is presented. A representative data set that shows live cell response to mechanical stimulation is presented.
10:48
Imaging Biological Samples with Optical and Confocal Microscopy
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2023
Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut Optical microscopes have been around for centuries, and while they reached their theoretical limitation of resolution decades ago, new equipment and techniques, such as confocal and digital image processing, have created new niches within the field of optical imaging. The best optical microscopes will typically have a resolution down to 200 nm in ideal...
14:58
Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters
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2010
We demonstrate a dark-field microscopy method based on Gabor-like filtering to measure subcellular dynamics within single living cells. The technique is sensitive to alterations in the structure of organelles, such as mitochondrial fragmentation.
FAQs
Improving spatial resolution reveals finer structural detail, whereas higher temporal resolution captures changes more frequently. Both choices can increase light exposure, which may disturb biological activity through phototoxicity. Imaging conditions therefore require a compromise tailored to the process being studied, so researchers can follow meaningful cellular or organismal changes without unnecessarily compromising specimen health.
Transmitted light and fluorescent light provide different ways to visualize living specimens. Transmitted light supports observation of overall structures and morphology, while fluorescence can reveal selected cellular features through emitted signals. The appropriate illumination depends on the specimen and experimental goal, because each approach contributes different information about structure and behavior during observation.
Living specimens must remain biologically active throughout image acquisition for sequential observations to represent natural change. Environmental control helps maintain suitable conditions, while limiting phototoxicity reduces light-related disruption. These considerations are especially important when experiments track prolonged processes such as movement, division, intracellular transport, or morphological change across multiple time points.
A typical workflow begins by selecting the living cell, tissue, or organism and choosing transmitted or fluorescent illumination according to the experimental aim. Optical lenses and a sensitive detector then capture sequential images under controlled imaging conditions. Researchers adjust the balance among detail, imaging frequency, light exposure, and specimen preservation before analyzing changes across the image sequence.
This approach is useful whenever biological behavior changes over time and cannot be understood from a single static image. It supports investigations of cell movement, division, intracellular transport, morphology, development, physiology, disease mechanisms, and responses to treatment. Its value comes from connecting visible structural changes with the timing and progression of biological events.
Sequential images can show when and how cells or organisms move, divide, transport material internally, or alter their morphology. Comparing images over time reveals the progression of these events rather than only their final appearance. In biology, that temporal information helps relate dynamic behavior to development, physiological processes, disease-related changes, or treatment responses.