Liver Mobilization
Liver Mobilization - Related Videos
12:35
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
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Cited by 14 •
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The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation.
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12:13
Ex Situ Normothermic Machine Perfusion of Donor Livers
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Cited by 21 •
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Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.
09:24
Decellularization and Recellularization of Whole Livers
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Cited by 39 •
2011
Perfusion decellularization is a novel technique to produce whole liver scaffolds that retains the organ's extracellular matrix composition and microarchitecture. Herein, the method of preparing whole organ scaffolds using perfusion decellularization and subsequent repopulation with hepatocytes is described. Functional and transplantable liver grafts can be generated using this technique.
FAQs
The operative benefit comes from releasing the liver’s ligamentous restraints so it can be repositioned away from the area being treated. This creates a clearer view of hepatic structures and nearby anatomy, while giving surgeons better control during complex procedures. The quality of exposure is especially important when working near major vessels or adjacent organs.
These ligaments form surrounding attachments that limit how the liver can be moved during surgery. Dividing the falciform, coronary, and triangular ligaments progressively releases those restraints and permits repositioning. Their coordinated division supports broader visualization of the liver and operative field, but the process must preserve the organ’s blood supply and biliary structures.
Repositioning the liver improves access only when its essential internal structures remain intact. Protecting the blood supply and biliary structures helps maintain hepatic integrity while the organ is moved for operative exposure. Careful handling also supports safer work around major vessels and adjacent organs, where inadequate control could complicate resection, trauma management, or other abdominal procedures.
By freeing the liver from selected attachments, the technique exposes relationships among hepatic tissue, surrounding ligaments, nearby organs, and major vessels. That improved access provides a practical foundation for examining hepatic anatomy in clinical research and surgical training. It also helps researchers and clinicians connect anatomical structure with the exposure requirements of hepatobiliary procedures.
The procedure begins by identifying the liver’s surrounding ligamentous attachments and then dividing the falciform, coronary, and triangular ligaments as needed. The released liver is repositioned to improve visibility and access to the operative field. Throughout these steps, surgeons maintain control of the blood supply and biliary structures and avoid injury to nearby organs and major vessels.
Surgeons use this technique as an exposure step in liver resection, transplantation, tumor removal, and trauma management. Its value varies with the operative objective, but the common purpose is to create sufficient access for complex work on or around the liver. Improved positioning can make the target area more visible and facilitate control during the procedure.
Careful mobilization can improve operative exposure, increase visibility of relevant anatomy, and help surgeons control the working field. It may also reduce the likelihood of injuring adjacent organs and major vessels by making their relationships easier to assess before and during treatment. These outcomes support more controlled management of complex hepatobiliary surgery.
In tumor removal, releasing the liver’s attachments can expose the region requiring treatment and support controlled surgical access. In trauma management, the same improvement in positioning and visibility can help clinicians assess and address injured hepatic areas. In both settings, the technique remains dependent on preserving the liver’s blood supply and biliary structures.