Luminescence Measurement
Luminescence Measurement - Related Videos
06:06
A Simple Dewar/Cryostat for Thermally Equilibrating Samples at Known Temperatures for Accurate Cryogenic Luminescence Measurements
0 Views •
2016
A simple liquid nitrogen Dewar/cryostat apparatus comprised of a small fused silica optical Dewar, a thermocouple, and a charge-coupled device (CCD) spectrograph are described. The experiments for which this Dewar/cryostat is designed require fast sample loading, freezing, and alignment, accurate and stable sample temperatures, and small size/portability.
02:36
The Luciferase Assay: A High-Throughput Assay to Measure Luminescent Signals Using Engineered Nanoluciferase System-Based Bioreporters
0 Views •
2025
The video demonstrates the luciferase assay used to quantify luciferase-tagged proteins. The assay uses an engineered luciferase enzyme composed of a large fragment that binds with the small fragment, which is conjugated with a protein of interest. The bound subunits form an active enzyme that, in the presence of the substrate, releases a luminescent signal. This signal is then quantified using a luminometer.
04:00
Luminescence-Based Assay to Assess Neurotoxicity of Test Compounds on Neurons
0 Views •
2025
In this video, we describe a luminescence-based method to assess the neurotoxicity of neurite outgrowth-promoting small molecule compounds on the viability of hNPC-derived neurons. The assay measures intracellular ATP level, an indicator of cell viability, to evaluate neurotoxicity following test compound treatment.
02:14
Expression of the Luminescence Operon in Marine Bioluminescent Bacteria
0 Views •
2025
Source: Brodl, E., et al. In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria. J. Vis. Exp. 1 (2018)This video demonstrates the cultivation of marine bioluminescent bacteria to observe quorum-sensing–mediated activation of the luminescence operon, enabling real-time monitoring of gene expression and light emission for studies in microbial communication and gene regulation.
07:04
A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)
0 Views •
Cited by 2 •
2014
Expanding the foundation and applicability of Fluorescence by Unbound Excitation from Luminescence (FUEL) by surveying the relevant principles and demonstrating its compatibility with a multitude of fluorophores and antibody-targeted conditions.
FAQs
Luciferase catalyzes a reaction in which its substrate is converted into an excited product. As that product returns to a lower-energy state, it releases photons. The instrument detects those photons as light intensity, linking the recorded signal to the biological reaction being studied and allowing researchers to quantify activity through relative or absolute measurements.
The enzyme and substrate form the reaction system that generates the detectable signal. Luciferase drives substrate conversion, while the resulting excited product produces photons. Changing the biological condition that controls this reaction can therefore alter the measured intensity, making the system useful for examining enzyme activity, reporter behavior, or other biological processes.
Relative intensity expresses the light signal as a comparative measurement, allowing conditions or samples to be evaluated against one another. Absolute intensity reports the emitted light quantitatively. This distinction affects how results are interpreted: relative values support comparisons within an experiment, whereas absolute measurements provide a direct quantitative description of emission.
Two important advantages are the small amount of sample required and the low background signal that can accompany the measurement. These features help researchers detect and compare light from biological reactions without using large sample volumes. As a result, luminescence-based assays can support sensitive analysis across gene expression, enzyme activity, and cellular studies.
A biological reaction or reporter system is first associated with a light-producing process, such as luciferase-mediated substrate conversion. The emitted light is then captured by an instrument and recorded as relative or absolute intensity. Researchers interpret that signal in relation to the biological process under investigation, including ATP content, cell viability, gene expression, or enzyme activity.
Researchers apply the technique when they need to monitor gene expression, ATP, cell viability, enzyme activity, or reporter systems. Its low sample requirement and low background signal also make it suitable for high-throughput analysis, where many biological samples or conditions must be assessed efficiently. These applications connect photon detection directly to quantitative biological readouts.