Macrophage Colony Stimulating Factor
Macrophage Colony Stimulating Factor - Related Videos
06:46
Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
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Cited by 58 •
2016
A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. The protocol utilizes cryopreservation of monocytes coupled with their bulk differentiation into macrophages. Then harvested macrophages can then be seeded into culture wells at required cell densities for carrying out experiments.
10:27
In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells
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Cited by 1 •
2018
Here, we present a protocol to differentiate murine granulocyte-macrophage-colony-stimulating-factor-producing T helper (THGM) cells from naive CD4+ T cells, including isolation of naive CD4+ T cells, differentiation of THGM, and analysis of differentiated THGM cells. This method can be applied to studies of the regulation and function of THGM cells.
12:02
Isolation of Exosome-Enriched Extracellular Vesicles Carrying Granulocyte-Macrophage Colony-Stimulating Factor from Embryonic Stem Cells
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Cited by 12 •
2021
This study describes a method to isolate exosome-enriched extracellular vesicles carrying immune-stimulatory granulocyte macrophage colony-stimulating factors from embryonic stem cells.
04:31
In Vitro Stimulation and Visualization of Macrophage Extracellular Traps
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2025
This video demonstrates in vitro stimulation of macrophage extracellular traps (METs) in human monocyte-derive macrophages using different inflammatory stimuli such as TNF-alpha, PMA, and HOCL. The video also demonstrates the method of SYTOX dye staining of METs for visualization.
09:23
Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion
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Cited by 19 •
2016
Understanding the malignant behavior of cancer requires creating accurate models of how tumor cells interact with components of the tumor microenvironment, such as macrophages. Here we describe two methods to study glioblastoma cell interaction with tumor associated macrophages and microglia where the effect on glioblastoma invasion is assessed.
FAQs
The key event is M-CSF binding to the CSF1 receptor on precursor and mature myeloid cells. This receptor engagement activates intracellular signals that support cell survival, proliferation, differentiation, and functional responses. Because these effects occur at different stages of myeloid development, the pathway can influence both the maintenance of developing cells and the behavior of macrophages after they mature.
These outcomes describe distinct ways that M-CSF signaling shapes myeloid populations. Survival preserves responsive cells, proliferation expands their number, and differentiation guides precursor cells toward macrophage development. Considering the outcomes separately helps researchers interpret whether M-CSF is maintaining a cell population, increasing its size, or changing its developmental state in a biological or experimental setting.
The pathway extends beyond immune-cell development because its signaling is also relevant to bone remodeling and the tumor microenvironment. This makes CSF1 biology useful for studying how macrophage-related processes intersect with tissue structure and disease-associated environments. Researchers can therefore examine the same signaling system in immune, skeletal, and tumor-focused biological questions.
M-CSF can be used with precursors obtained from bone marrow or blood to generate macrophages for laboratory studies. The choice of source allows investigators to establish macrophage systems from different precursor populations while examining their development under controlled research conditions. These generated cells provide an experimental basis for studying innate immunity, inflammation, infection, or tissue repair.
M-CSF-generated macrophages support research on innate immunity, inflammation, infection, and tissue repair. They provide a defined cellular system in which investigators can examine macrophage development and function in relation to these processes. The approach is especially useful when researchers need macrophages as an experimental model rather than relying only on observations from complex tissues.
M-CSF is relevant across research areas because its signaling affects myeloid-cell development while also connecting to bone remodeling and the tumor microenvironment. Basic studies can use the pathway to examine macrophage biology, whereas disease-oriented work can investigate its role in tissue and tumor contexts. These links make CSF1 signaling a subject of therapeutic research as well as fundamental biology.