Micromanipulation Microscope

A micromanipulation microscope is an optical instrument that combines high-magnification imaging with precise mechanical control to manipulate cells, tissues, or other microscopic specimens. It works by pairing a microscope with micromanipulators that guide fine tools, such as needles, pipettes, or electrodes, while the operator monitors movement through the viewing system. In biology, this setup supports procedures including cell injection, embryo handling, biopsy, and targeted sampling, where accuracy and minimal physical disturbance are essential. Micromanipulation microscopes help researchers perform controlled experiments on individual cells and structures, advancing studies of fertilization, development, cell function, and disease mechanisms.

Micromanipulation Microscope - Related Videos

Research

JoVE EoE - Prostate Cancer

Single Cell Sampling Using Micromanipulation: A Technique to Isolate Single Cells from Cancer Cell Suspension

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2023

This video describes the technique for isolating single cells from a pool of target cells to carry out downstream analysis. In this method, a micromanipulator fitted to a microscope is used for high-precision isolation of a single target cell.

Micromanipulation of Chromosomes in Insect Spermatocytes

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Cited by 9 •

2018

In this protocol, we describe the selection and preparation of appropriate cells for micromanipulation and the use of a piezoelectric micromanipulator to reposition chromosomes within those cells.

Research

JoVE Journal - Biology
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Microiontophoresis and Micromanipulation for Intravital Fluorescence Imaging of the Microcirculation

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Cited by 3 •

2011

Microiontophoresis entails movement of ions from a micropipette in response to a difference in electrical potential between the inside and outside of the micropipette. Biologically active molecules are thereby delivered in proportion to electrical current. We illustrate acetylcholine microiontophoresis in conjunction with micromanipulation to study endothelium-dependent vasodilation in the microcirculation.

Research

JoVE Journal - Neuroscience
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Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides

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Cited by 46 •

2013

In this article, we demonstrate a method for manipulation of gene expression in the ventricular cells of the adult zebrafish telencephalon using antisense morpholino oligonucleotides. We present this method as an efficient and quick protocol that can be used for functional studies in the adult vertebrate brain.

Research

JoVE Journal - Biology
Free Sample

Proper Care and Cleaning of the Microscope

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Cited by 2 •

2008

Keeping the microscope optics clean is important for high-quality imaging. Dust, fingerprints, excess immersion oil, or mounting medium on or in a microscope causes reduction in contrast and resolution. DIC is especially sensitive to contamination and scratches on the lens surfaces. This protocol details the procedure for keeping the microscope clean.

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