Micropipette Fabrication
Micropipette Fabrication - Related Videos
10:31
Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
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Cited by 30 •
2012
Here we describe a quick and simple method to measure cell stiffness. The general principle of this approach is to measure membrane deformation in response to well-defined negative pressure applied through a micropipette to the cell surface. This method provides a powerful tool to study biomechanical properties of substrate-attached cells.
07:36
Fabricating Nanogaps by Nanoskiving
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Cited by 3 •
2013
The fabrication of electrically addressable, high-aspect-ratio (> 1000:1) metal nanowires separated by gaps of single nanometers using either sacrificial layers of aluminum and silver or self-assembled monolayers as templates is described. These nanogap structures are fabricated without a clean room or any photo- or electron-beam lithographic processes by a form of edge lithography known as nanoskiving.
08:29
Fabrication of Amperometric Electrodes
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Cited by 8 •
2009
This protocol describes how to generate carbon fiber electrodes. The electrodes are subsequently used to detect catecholamine release from vesicles with carbon fiber amperometry.
05:40
Fabricating Cotton Analytical Devices
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2016
To investigate simple fabrication approaches for multiple assay needs, we created a fluid-absorbing channel system made of cotton material. This device was used to establish a multiple detection platform, and solve contamination issues that commonly affect lateral flow-based biomedical devices, for clinical urinalysis of nitrite, total protein, and urobilinogen.
10:26
Fabrication and Characterization of Superconducting Resonators
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Cited by 3 •
2016
Superconducting microwave resonators are of interest for detection of light, quantum computing applications and materials characterization. This work presents a detailed procedure for fabrication and characterization of superconducting microwave resonator scattering parameters.
FAQs
Controlled heating softens the glass capillary, while tension draws the softened material into a narrower form. The glass type, heat, pulling force, and cooling conditions collectively influence the resulting tip diameter, taper, and overall shape. Adjusting these variables allows researchers to produce tools suited to different requirements for cell manipulation, fluid handling, or contact with cellular membranes.
Tip geometry determines how the pipette interacts with cells and microscopic volumes. A fine, appropriately tapered tip can support controlled penetration, fluid delivery, aspiration, or stable membrane contact, whereas an unsuitable shape may limit control. Consequently, fabrication parameters are selected according to whether the pipette will be used for microinjection, cell aspiration, electrophysiology, or patch-clamp recording.
Beveling or polishing can be performed after pulling to modify the finished tip. These treatments are used when a particular edge or surface is needed to improve penetration or fluid control. Such refinement is especially relevant when the pipette must enter a cell, draw material from it, or establish consistent contact with a membrane during biological measurements.
Fabrication begins with a glass capillary placed under controlled heating and tension. As the glass softens, pulling causes it to elongate until it separates into fine-tipped pipettes. Researchers can then bevel or polish the tips when additional refinement is needed. The final geometry reflects the selected glass, heating, pulling, and cooling conditions.
Fabricated micropipettes support several complementary techniques, including microinjection, cell aspiration, electrophysiology, and patch-clamp recording. In microinjection, they help deliver reagents; in aspiration, they manipulate cellular material. Electrophysiology and patch-clamp experiments use their geometry to support controlled interaction with cells and membranes while researchers measure cellular properties or establish stable contact.
Their applications extend beyond physical manipulation. Micropipettes can deliver reagents into cells, remove or handle cellular material, and help establish the membrane contact required for recording. Depending on the experiment, researchers may therefore obtain information about cellular properties, perform targeted molecular delivery, or control interactions with individual cells and microscopic volumes.