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Fabrication of Amperometric Electrodes
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JoVE Journal Biology
Fabrication of Amperometric Electrodes

Fabrication of Amperometric Electrodes

DOI:
10.3791/1040-v

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08:29 min

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May 04, 2009

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, ,
1Department of Pharmacological and Physiological Science,Saint Louis University School of Medicine, 2Yale University School of Medicine

Chapters

  • 00:04Title
  • 00:54Introduction
  • 01:20Carbon Fiber Preparation
  • 02:23Fire-Polishing Glass Capillary Tubes
  • 03:13Threading a Carbon Fiber into a Capillary Tube
  • 03:48Pulling Pipette-Fiber in an Electrode Puller
  • 04:43Making Epoxy-Glass Seal
  • 06:23Cutting the Carbon Fiber
  • 07:25Representative Results
  • 08:04Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to generate carbon fiber electrodes. The electrodes are subsequently used to detect catecholamine release from vesicles with carbon fiber amperometry.

Tags

Amperometric ElectrodesCarbon Fiber ElectrodesDetectionCatecholamine ReleaseVesiclesSingle CellsAmperometry MeasurementsLow Noise ElectrodesCarbon FibersCapillary TubeVacuum AspirateElectrode PullerEpoxy-glass SealOven CuringFabricationCutting Amperometric Electrodes

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