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JoVE Journal
Bioengineering

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Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
 

Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness

Article doi: 10.3791/3886
September 27th, 2012

Summary September 27th, 2012

Here we describe a quick and simple method to measure cell stiffness. The general principle of this approach is to measure membrane deformation in response to well-defined negative pressure applied through a micropipette to the cell surface. This method provides a powerful tool to study biomechanical properties of substrate-attached cells.

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