Microscopy Advances
Microscopy Advances - Related Videos
11:26
Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
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Cited by 8 •
2009
Here we demonstrate the protocols for performing single-molecule fluorescence microscopy on living bacterial cells to enable functional molecular complexes to be detected, tracked and quantified.
09:59
Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
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Cited by 3 •
2013
Autophagy is a ubiquitous process that enables cells to degrade and recycle proteins and organelles. We apply advanced fluorescence microscopy to visualize and quantify the small, but essential, physical changes associated with the induction of autophagy, including the formation and distribution of autophagosomes and lysosomes, and their fusion into autolysosomes.
10:19
Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy
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2019
An advanced microscope that permit fast and high-resolution imaging of both, the isolated plasma membrane and the surrounding intracellular volume, will be presented. The integration of spinning disk and total internal reflection fluorescence microscopy in one setup allows live imaging experiments at high acquisition rates up to 3.5 s per image stack.
08:52
Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice
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Cited by 32 •
2018
This protocol describes the intravital imaging of transgenic mice expressing cell-specific fluorescent markers. Intravital imaging provides a non-invasive method for high-resolution observations in living animals using implantable windows through which the microscopic visualization of processes is possible. This method is especially useful to study longitudinal processes.
12:43
Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions
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Cited by 8 •
2017
Laser microirradiation is a useful tool for studies of DNA repair in living cells. A methodological approach for the use of UVA lasers to induce various DNA lesions is shown. We have optimized a method for local microirradiation that maintains the normal cell cycle; thus, irradiated cells proceed through mitosis.
FAQs
Illumination controls how biological specimens are exposed to light, while image acquisition determines how the resulting signal is captured. Adjusting these elements can improve contrast, increase imaging speed, or reduce disruption during observation. These changes are especially important when researchers need to follow cellular events over time rather than obtain only a static image.
Electron-based methods use accelerated electrons to reveal cellular ultrastructure beyond the limits of conventional optical microscopy. Advanced optical approaches instead manipulate light, fluorescence, illumination, and acquisition to examine structures or processes with improved contrast, speed, or molecular specificity. The distinction helps researchers match the imaging method to questions about fine structure versus dynamic biological behavior.
Computational reconstruction processes acquired image data to enhance or quantify what the microscope records. Rather than serving only as a display step, it can support analysis of biological structures and measurements within images. This capability is valuable when researchers need three-dimensional tissue mapping or quantitative interpretation alongside direct visualization.
Method selection should follow the information the experiment requires: resolution for fine structures, contrast for distinguishing features, speed for dynamic events, or molecular specificity for identifying particular biological components. Researchers must also consider whether the sample needs live observation or minimal disruption. These criteria connect instrument choice with the biological question and the expected outcome.
Live-cell imaging uses improved imaging capabilities to observe biological events as they occur, while super-resolution analysis addresses structures that conventional optical microscopy cannot distinguish adequately. Together, these applications help connect cellular behavior with underlying molecular mechanisms. Their value lies in examining both temporal change and fine spatial organization within biological systems.
Three-dimensional tissue mapping provides spatial information about biological structures and their organization within tissues. When combined with improved resolution, contrast, or molecular specificity, imaging can help characterize cellular components and relate them to cell behavior. In disease research, these observations support efforts to connect molecular mechanisms with altered cellular or tissue-level processes.