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1Department of Biochemistry and Molecular Medicine,University of California, Davis , 2NSF Center for Biophotonics Science & Technology,University of California, Davis , 3University of Tromsø, 4Department of Surgery (Division of Surgical Oncology),University of California, Davis , 5UC Davis Comprehensive Cancer Center,University of California, Davis , 6Department of Biological Chemistry,University of California, Davis
Chapters
- 00:05Title
- 01:27Preparing Live Cell Specimens for Imaging
- 03:103D (Deconvolution) Fluorescence Microscopy
- 06:10Results: Quantitative Image-Based Analysis of Cells Undergoing Autophagy
- 09:02Conclusion
Autophagy is a ubiquitous process that enables cells to degrade and recycle proteins and organelles. We apply advanced fluorescence microscopy to visualize and quantify the small, but essential, physical changes associated with the induction of autophagy, including the formation and distribution of autophagosomes and lysosomes, and their fusion into autolysosomes.
