Journal
/
Bioengineering
/
Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

DOI:
10.3791/58756-v

•

10:19 min

•

January 21, 2019

•

,
1UKE Microscopy Imaging Facility,University Medical Center Hamburg-Eppendorf

Chapters

  • 00:04Title
  • 01:15Cell Preparation
  • 04:12Live Cell Imaging
  • 06:50Image Post-processing in ImageJ
  • 08:14Results: Cell Spreading and Adhesion Formation using SpinningDisk-TIRF Microscopy
  • 09:13Conclusion

Summary

Automatic Translation

Automatic Translation

An advanced microscope that permit fast and high-resolution imaging of both, the isolated plasma membrane and the surrounding intracellular volume, will be presented. The integration of spinning disk and total internal reflection fluorescence microscopy in one setup allows live imaging experiments at high acquisition rates up to 3.5 s per image stack.

Tags

Keywords: Spinning DiskTIRF MicroscopyCytoskeletal NetworkPlasma MembraneLive ImagingCell BiologyMicrobiologyFluorescence MicroscopyRFP LivactYFP VinculinFibronectinMulti-fluorescent Beads

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58756?article_type=v

Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition
check_url/58756?article_type=v

Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy
check_url/58756?article_type=v

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy (VA-TIRFM)
check_url/58756?article_type=v

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
check_url/58756?article_type=v

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
check_url/58756?article_type=v

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
check_url/58756?article_type=v

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
check_url/58756?article_type=v

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair
check_url/58756?article_type=v

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy
check_url/58756?article_type=v

Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy

Read Article