Molecular Detection
Molecular Detection - Related Videos
08:56
In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
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Cited by 4 •
2012
We demonstrate the assembly and application of a molecular-scale device powered by a topoisomerase protein. The construct is a bio-molecular sensor which labels two major types of DNA breaks in tissue sections by attaching two different fluorophores to their ends.
05:38
Rapid Molecular Detection and Differentiation of Influenza Viruses A and B
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Cited by 4 •
2017
We describe a rapid, molecular-based Influenza A and B assay. The Influenza assay detects each target within 15 min by employing isothermal amplification with influenza-specific primers followed by target detection with molecular beacon probes. The Influenza A and B assay is user-friendly and required minimal hands-on time to perform.
08:49
Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein
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Cited by 1 •
2011
We describe a molecular clone of maedi-visna virus that expresses GFP and is fully infectious. Replication of this virus can be detected by using fluorescence microscopy and flow cytometry.
06:54
MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent
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Cited by 3 •
2013
To demonstrate MR cancer molecular imaging with a small peptide targeted MRI contrast agent specific to clotted plasma proteins in tumor stroma in a mouse prostate cancer model.
03:07
Kinetic Molecular Theory: Molecular Velocities, Temperature, and Kinetic Energy
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2020
The kinetic molecular theory qualitatively explains the behaviors described by the various gas laws. The postulates of this theory may be applied in a more quantitative fashion to derive these individual laws. Collectively, the molecules in a sample of gas have average kinetic energy and average speed; but individually, they move at different speeds. Molecules frequently undergo elastic collisions in which the momentum is conserved. Since the colliding molecules are deflected off at different...
FAQs
Complementary binding allows a detection system to recognize a target based on matching molecular features, particularly in nucleic acids. This selectivity helps distinguish the intended biological molecule from other components in a complex sample. As a result, researchers can associate the measured signal more confidently with a pathogen, genetic change, or other targeted biological process.
Amplification methods increase the amount of detectable target or the signal associated with it. This improves sensitivity when the original sample contains only a small quantity of a nucleic acid or another relevant molecule. Greater sensitivity can support earlier recognition of biological changes and improve the ability to detect targets within complex biological samples.
The target determines which aspect of biology the analysis can reveal. Nucleic acids can indicate genetic information or changes, proteins can reflect molecular activity or cellular characteristics, and metabolites can provide information about biochemical states. Selecting among these molecule types therefore connects the measurement to questions about genes, cell activity, pathogens, or biomarkers.
A general workflow begins by selecting a biological molecule linked to the question being studied. The sample is then assessed using complementary binding, selective chemical interactions, or a signal-generating reaction, with amplification added when greater sensitivity is needed. The resulting signal or measurement is interpreted in relation to the suspected cell, pathogen, genetic change, or biomarker.
In pathogen diagnosis, measuring a pathogen-associated molecule can reveal its presence in a biological sample. For genetic analysis, the same overall approach can identify gene expression patterns or mutations. These applications provide molecular-level evidence rather than relying only on broader sample characteristics, supporting more precise investigation of infection and genetic changes.
Biomarker research uses molecular measurements to examine molecules associated with biological states or processes. Environmental monitoring can apply the same principle to identify biological molecules indicating the presence or activity of organisms or pathogens in environmental samples. Because these methods analyze targets within complex samples, they help connect molecular findings with broader biological conditions.