Non-viral T Cell Engineering
Non-viral T Cell Engineering - Related Videos
21:08
Engineering Cell-permeable Protein
0 Views •
Cited by 12 •
2009
Protein transduction enables the direct delivery of biologically active proteins into cells. In contrast to conventional methods such as DNA transfection or viral transduction this non-invasive paradigm allows highly efficient cellular manipulation in a titratable manner circumventing cellular toxicity and the risk of oncogenic transformation by permanent genetic modification.
06:10
Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
0 Views •
2025
A detailed protocol is provided for using CRISPR/Cas9 technology to achieve highly efficient targeted knock-in of large, multicistronic constructs in primary human T cells via the homology-mediated end joining (HMEJ) DNA repair pathway. T cells engineered with this cGMP-adaptable protocol maintain excellent cell expansion, cytotoxicity, and cytokine production.
04:42
Micro-scale Engineering for Cell Biology
0 Views •
2007
02:38
An Assay to Evaluate the Effect of Test Compounds on Viral Entry and Fusion in Host Cells
0 Views •
2025
This video demonstrates an assay to evaluate antiviral agents that target specific steps of the viral entry into host cells and fusion with the endosome. The cells are incubated at a low temperature and then infected with HCV. The low temperature permits viral binding to host cells but inhibits internalization via endocytosis. The test antiviral compound is added, and the cells are incubated at physiological temperature. This shift to a higher temperature facilitates viral entry and allows...
14:43
Synthesis of Keratin-based Nanofiber for Biomedical Engineering
0 Views •
Cited by 12 •
2016
Electrospun nanofibers have a high surface area to weight ratio, excellent mechanical integrity, and support cell growth and proliferation. These nanofibers have a wide range of biomedical applications. Here we fabricate keratin/ PCL nanofibers, using the electrospinning technique, and characterize the fibers for possible applications in tissue engineering.
FAQs
The delivered payload determines both the duration and type of change. DNA or messenger RNA can provide instructions for protein production, whereas genome-editing components are used when the goal is a targeted genomic change. This distinction lets investigators choose transient expression for rapid experiments or more durable genetic modification when studying engineered T-cell function.
Compared with viral vector-based production, non-viral T cell engineering offers an alternative route for introducing instructions without relying on viral vector production. Its value lies in platform flexibility: investigators can work with DNA, messenger RNA, or editing components and select physical or chemical delivery approaches. This supports rapid experiments and evaluation of next-generation treatments.
Electroporation, lipid-based systems, and other physical or chemical carriers influence how molecular instructions reach T lymphocytes. Their shared purpose is delivery, but the selected platform can be matched to the intended payload and experimental goal. This choice is important when researchers need transient protein expression, targeted genomic changes, or flexible cell-production workflows.
A conceptual workflow starts by selecting the desired genetic or molecular instruction, such as DNA, messenger RNA, or genome-editing components. Researchers then pair that payload with a suitable physical or chemical delivery approach, including electroporation or lipid-based systems. The engineered cells can subsequently be evaluated for protein expression, genomic changes, or altered immune-cell function.
Transient experiments are useful when researchers want rapid evaluation of protein expression or engineered T-cell behavior without focusing exclusively on durable genomic alteration. Messenger RNA can support this type of short-term investigation, while the broader non-viral platform enables flexible testing of functional proteins. These studies help evaluate candidate designs and next-generation treatment concepts.
In biology and immunotherapy research, engineered T cells can be designed to express chimeric antigen receptors, modified T cell receptors, or other functional proteins. These cells provide systems for studying immune responses and investigating potential cell therapies. The approach also supports evaluation of next-generation treatments by enabling rapid experiments and alternative manufacturing strategies.