Molecular Extraction Procedure
Molecular Extraction Procedure - Related Videos
09:30
Extraction of High Molecular Weight DNA from Microbial Mats
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Cited by 4 •
2011
We provide an improved protocol for extracting high molecular weight DNA from hypersaline microbial mats. Microbial cells are separated from the mat matrix prior to DNA extraction and purification. This enhances the concentrations, quality, and size of the DNA. The protocol may be used for other refractory samples.
11:24
Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
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Cited by 21 •
2009
A methodology to isolate high molecular weight and high quality genomic DNA from soil microbial community is described.
10:37
Procedure to Evaluate the Efficiency of Flocculants for the Removal of Dispersed Particles from Plant Extracts
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Cited by 6 •
2016
The design-of-experiments procedure presented here allows the evaluation of different flocculants in terms of their ability to aggregate dispersed particles in plant extracts, thus reducing turbidity and the costs of downstream processing.
03:22
SDS-PAGE Based Extraction of Extracellular Vesicles Associated Proteins: A Procedure to Extract Proteins from EVs and Prepare Them for In-Gel Digestion
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2023
In this video, we demonstrate the SDS-PAGE in-gel digestion method for protein extraction from extracellular vesicles or EVs. Once isolated, the obtained protein fragments can be used for proteomic analysis.
09:55
Molecular Cloning
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2023
Molecular cloning is a set of methods, which are used to insert recombinant DNA into a vector - a carrier of DNA molecules that will replicate recombinant DNA fragments in host organisms. The DNA fragment, which may be a gene, can be isolated from a prokaryotic or eukaryotic specimen. Following isolation of the fragment of interest, or insert, both the vector and insert must be cut with restriction enzymes and purified. The purified pieces are joined together though a technique called...
FAQs
Cell or tissue lysis releases the biomolecules that later purification steps must isolate. Detergents disrupt membranes, while enzymes can help break down biological structures or contaminants; salts can support subsequent separation. This stage affects recovery and integrity, so conditions should be selected according to the sample and target molecule rather than applied identically to every biological specimen.
After lysis, contaminants are removed through selective separation and purification rather than by lysis alone. Centrifugation can help separate membranes, proteins, and other unwanted material, while precipitation or solid-phase binding provides additional purification routes. Choosing among these approaches depends on the sample, the molecule being recovered, and whether downstream work requires especially strong preservation of molecular integrity.
Suitability depends mainly on sample type, target molecule, and the degree of integrity required for the next experiment. Conditions that work for DNA may not be appropriate when the goal is RNA or protein analysis, because each target has different preservation needs. Quality-control methods then help determine whether the recovered material is appropriate for downstream analysis or use.
A typical workflow starts by lysing the biological sample, then separates the released material from membranes, proteins, and other contaminants. Purification follows through precipitation or solid-phase binding, with centrifugation used where appropriate. The final stage is quality control, which checks whether the isolated DNA, RNA, protein, or other biomolecule has the quality needed for PCR, sequencing, expression analysis, or another downstream experiment.
Common components include detergents, enzymes, salts, centrifugation equipment, precipitation reagents, and solid-phase materials. Their roles are complementary: lysis reagents open the sample, separation tools and reagents help remove unwanted material, and purification components support recovery of the selected biomolecule. The exact combination is determined by sample type, target molecule, and required molecular integrity.
Extracted biomolecules provide the input for many biological analyses, including polymerase chain reaction, sequencing, gene expression analysis, proteomics, and diagnostic testing. The extraction strategy influences whether the target is sufficiently purified and intact for those applications. Thus, the procedure connects biological sampling with molecular measurements, while quality control helps determine whether the preparation can support a reliable downstream result.