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Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
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Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

Article DOI: 10.3791/1569 • 11:24 min • November 10th, 2009
November 10th, 2009 •
1, 1
1Department of Microbiology and Immunology, University of British Columbia - UBC

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Summary
Automatic Translation

A methodology to isolate high molecular weight and high quality genomic DNA from soil microbial community is described.

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High Molecular Weight Genomic DNA Soils Sediments Soil Microbiome Metagenomic Approaches Genetic Information Pathway Reconstruction Functional Screening Therapeutic Processes Biomass Conversion Processes Large Insert Library Production Protocol Cell Lysis Mechanical Forces Chemical Forces Genomic DNA Extraction Extraction Buffer Chloroform-isoamyl Alcohol Isopropyl Alcohol Guanidine Isothiocyanate Hexadecyltrimethylammonium Bromide (CTAB) Integrity Preservation
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