Mosquito Embryo Microinjection
Mosquito Embryo Microinjection - Related Videos
17:39
Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes
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Cited by 56 •
2007
In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing microinjection needles, dessicating embryos, and performing microinjection are demonstrated.
03:12
Mosquito Embryo Microinjection: A Technique to Deliver Exogenous DNA into Embryo Yolk of Anopheles Gambiae to Generate Germline Mutations
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2025
In this video, we demonstrate the delivery of gene drive construct into the embryo of Anopheles gambiae mosquito through embryo microinjection technique. This procedure allows for integration of delivered DNA construct, through homologous recombination, into the germline cells to generate transformed mosquito population.
06:54
Injection of An. stephensi Embryos to Generate Malaria-resistant Mosquitoes
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Cited by 9 •
2007
Anopheles stephensi mosquitoes are vectors for malaria inhabiting India and throughout Asia. This video demonstrates the technique for performing microinjections of this species with transgenes that will confer resistance to the malaria to the mosquito. Much of the methodology demonstrated in this video is applicable to microinjection techniques of other mosquito species.
05:51
Microinjection of Live Drosophila Embryos: Early Delivery of Reagents to the Developing Embryo
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2023
This video describes how to perform microinjections that deliver reagents into live Drosophila embryos. Researchers can use this method to introduce exogenous compounds like nucleic acids and proteins during the early stages of embryo development. The featured protocol demonstrates how to set up and perform the procedure for injection of any soluble reagent–here, a fluorescent protein for live imaging experiments.
03:10
Microinjection of Bacteria into the Caudal Vein of Zebrafish Embryos
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2026
Source: Shi, J., et al., Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens. J. Vis. Exp. (2020)This video demonstrates the procedure for preparing an Agrobacterium tumefaciens culture by inducing virulence gene expression with acetosyringone, followed by antibiotic removal through washing and resuspension, to generate a transformation-ready suspension for gene delivery into plant tissues.
FAQs
Timing matters because the injection occurs shortly after eggs are laid, while the embryo is beginning early development. Material introduced at this stage can remain present as cells divide, creating an opportunity for CRISPR components to direct targeted DNA changes. This early intervention also supports the possibility that altered genetic information reaches the developing germline.
Once delivered into the embryo, CRISPR components can direct targeted changes in DNA rather than acting only as nonspecific reagents. Their activity is linked to the embryo’s ongoing cell divisions, so the resulting genetic modification can become associated with developing tissues and, when the germline is established, support studies of inherited genetic effects.
The chorion, which surrounds the mosquito egg, forms the barrier that the injected material must cross to reach the embryo. A fine glass needle provides the physical route for penetration and deposition. The combination of a defined entry point and precise delivery allows researchers to introduce genetic material or other reagents during a narrowly selected developmental window.
A basic workflow begins with eggs shortly after they are laid, followed by positioning a fine glass needle to penetrate the chorion. The needle then deposits genetic material or another reagent into the developing embryo. When CRISPR components are used, the subsequent developmental process provides the setting in which targeted DNA changes may occur as cells divide.
This technique supports investigations of gene function and mosquito development by allowing researchers to introduce material during early embryogenesis. It also contributes to studies of vector competence, meaning the mosquito’s role in supporting disease transmission, and mosquito-borne disease biology. These applications connect precise genetic manipulation with questions about development and disease-related traits.
By enabling targeted genetic changes during early mosquito development, the method can support research programs aimed at population modification. Such work examines how genetic manipulation might contribute to broader disease-control strategies. The technique therefore links embryo-scale laboratory intervention with larger questions about mosquito populations and their importance in the biology of mosquito-borne diseases.