Injection of An. stephensi Embryos to Generate Malaria-resistant Mosquitoes

Olle Terenius; Jennifer Juhn; Anthony A. James

doi:10.3791/216

Journal

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Biology

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Injection of An. stephensi Embryos to Generate Malaria-resistant Mosquitoes

JoVE Journal
Biology

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JoVE Journal Biology

Injection of An. stephensi Embryos to Generate Malaria-resistant Mosquitoes

12,944 Views

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06:54 min

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July 04, 2007

DOI:

10.3791/216-v

DOI:

10.3791/216-v

•

06:54 min

July 04, 2007

•

3 Views

Olle Terenius¹, Jennifer Juhn¹, Anthony A. James²

¹Department of Molecular Biology and Biochemistry,University of California, Irvine (UCI), ²Department of Molecular Biology and Biochemistry, Department of Microbiology and Molecular Genetics,University of California, Irvine (UCI)

Summary

Anopheles stephensi mosquitoes are vectors for malaria inhabiting India and throughout Asia. This video demonstrates the technique for performing microinjections of this species with transgenes that will confer resistance to the malaria to the mosquito. Much of the methodology demonstrated in this video is applicable to microinjection techniques of other mosquito species.