Dengue Virus Infection
Dengue Virus Infection - Related Videos
15:25
Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination
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Cited by 35 •
2007
Once a gene is identified as potentially refractory for the dengue virus, it must be evaluated for it's role in preventing viral infections within the mosquito. This protocol illustrates how the extent of dengue infections of mosquitoes can be assayed. The techniques for growing up the virus in culture, membrane feeding mosquitoes human blood, and assaying viral titers in the mosquito midgut are demonstrated.
09:11
Visualizing Dengue Virus through Alexa Fluor Labeling
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Cited by 12 •
2011
Taking advantage of the advancements in fluorophore development and imaging technology, a simple method of Alexa Fluor labeling of dengue virus was devised to visualize the early interactions between virus and cell.
03:18
Antibody-Dependent Enhancement of Zika Virus by Dengue Antibodies in Human Primary Macrophages
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2026
Source: Asad, S., et al. Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells. J. Vis. Exp. (2019)This video demonstrates antibody-dependent enhancement of Zika virus infection in human primary macrophages, using dengue antibody–virus complexes to model how cross-reactive antibodies facilitate Fcγ receptor–mediated viral entry and replication for studying infection mechanisms and immune interactions.
08:36
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
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Cited by 7 •
2018
Real-time quantitative polymerase chain reaction analysis combined with reverse transcription (RT-qPCR) has been widely used to measure the level of RNA virus infections. Here we present a direct RT-qPCR assay, which does not require an RNA purification step, developed for the quantification of several RNA viruses, including dengue virus.
17:50
Preventing the Spread of Malaria and Dengue Fever Using Genetically Modified Mosquitoes
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Cited by 10 •
2007
In this candid interview, Anthony A. James explains how mosquito genetics can be exploited to control malaria and dengue transmission. Population replacement strategy, the idea that transgenic mosquitoes can be released into the wild to control disease transmission, is introduced as well as the concept of genetic drive and the design criterion for an effective genetic drive system. The ethical considerations of releasing genetically-modified organisms into the wild are also discussed.
FAQs
The four serotypes are closely related but immunologically distinct. An immune response generated against one serotype may not provide complete protection against another, making the history of previous infection important when interpreting disease mechanisms and evaluating vaccines. This serotype diversity helps explain why dengue research must consider cross-serotype immunity rather than treating all infections as identical.
Antibody-dependent enhancement occurs when antibodies produced after an earlier infection interact with a different dengue serotype in a way that can enhance infection rather than fully neutralize it. This mechanism links prior immune history to viral pathogenesis and helps explain why antibody responses require careful study when assessing disease severity, vaccine performance, or antiviral strategies.
Dengue activates both innate and adaptive immune responses after entering susceptible cells and replicating. Innate immunity provides an early response to infection, while adaptive immunity develops more targeted antibody and cellular responses. Studying the interaction between these systems is important because immune activity contributes to disease biology and helps researchers interpret protective responses alongside mechanisms that may worsen infection.
The clinical range reflects interactions among viral replication, the infected host cells, and immune responses. Differences among dengue serotypes and the presence of antibodies from a prior infection can alter these interactions, including through antibody-dependent enhancement. Examining these processes allows biology researchers to connect cellular events and immune mechanisms with variation in disease severity.
A study may focus on how dengue enters susceptible cells, replicates, and activates innate and adaptive immunity. Researchers also examine diagnostic markers, vaccine performance, and antiviral strategies to connect molecular and cellular observations with disease biology. Together, these areas provide a framework for investigating pathogenesis and for identifying approaches that could improve detection, prevention, or treatment.
Diagnostic markers provide measurable evidence that helps researchers and healthcare systems identify or characterize dengue virus infection. Their study connects the presence of infection with the underlying processes of viral replication and immune activation. In biology research, marker analysis can also support comparisons among cases, improve disease surveillance, and supply information needed to evaluate prevention or treatment strategies.
Dengue has four closely related serotypes, and immunity to one does not necessarily represent the same immune context as immunity to another. Vaccine performance therefore needs to be interpreted in relation to serotype diversity and the possibility of antibody-dependent enhancement. This perspective helps researchers assess whether an intervention produces useful protection across the biological conditions relevant to dengue infection.
Because infected Aedes mosquitoes transmit dengue, surveillance can help public-health programs track infection and transmission patterns. Control measures can then focus on reducing opportunities for mosquito-mediated spread. These activities complement laboratory research on pathogenesis, diagnostics, vaccines, and antivirals by applying biological knowledge to population-level prevention and lowering the risk of continued transmission.