Mouse Edl
Mouse Edl - Related Videos
03:53
Myofiber Isolation: A Technique to Obtain Single Myofibers from Harvested Mouse Extensor Digitorum Longus (EDL) Muscle
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2025
This video demonstrates the isolation of single myofibres from harvested murine Extensor Digitorum Longus (EDL) muscle. The isolated myofibres can be cultured to study myofiber-associated muscle stem cell activity.
09:08
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
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Cited by 59 •
2011
Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.
07:04
A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse
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Cited by 5 •
2017
Mice are widely used to study gestational biology. However, pregnancy termination is required for such studies which precludes longitudinal investigations and necessitates the use of large numbers of animals. Therefore, we describe a non-invasive technique of high-frequency ultrasonography for early detection and monitoring of post-implantation events in the pregnant mouse.
07:46
In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
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Cited by 39 •
2010
Orthotopic animal models of ovarian cancer replicate better human disease and therefore enhance our understanding of cancer progression and tumor response to therapy. A mouse model receives an intrabursal injection of luciferase-expressing ovarian tumor cells. Treatment is administered via oral gavage. Tumor growth is monitored by in vivo imaging system.
14:36
Evaluation of Muscle Function of the Extensor Digitorum Longus Muscle Ex vivo and Tibialis Anterior Muscle In situ in Mice
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Cited by 75 •
2013
Changes in limb muscle contractile and passive mechanical properties are important biomarkers for muscle diseases. This manuscript describes physiological assays to measure these properties in the murine extensor digitorum longus and tibialis anterior muscles.
FAQs
An action potential initiates calcium release from the sarcoplasmic reticulum, increasing calcium available to regulatory proteins. Calcium binding changes the regulatory state of the contractile apparatus, permitting actin-myosin cross-bridge cycling. The resulting interactions convert excitation into measurable muscle force, making calcium handling central to EDL contractile analysis.
Predominance of fast-twitch fibers makes the EDL useful for examining how a muscle with this fiber profile produces force and responds to stimulation. Consequently, measurements of contractile strength, fatigue, excitation-contraction coupling, and recovery can be interpreted in relation to fast-twitch muscle behavior rather than treated as generic properties of every skeletal muscle.
Fatigue describes a decline in contractile performance during or after stimulation, whereas recovery describes the return of performance afterward. Considering both outcomes helps distinguish an immediate reduction in force-generating capacity from the muscle’s subsequent restoration. This pairing is useful when evaluating muscle function, injury, aging, or therapeutic interventions.
Under controlled conditions, stimulation of an isolated EDL allows researchers to assess contractile strength and follow changes associated with fatigue and recovery. The same preparation can also be used to examine excitation-contraction coupling, linking electrical activation, calcium release, and force production. Together, these outcomes provide functional readouts of muscle performance.
Researchers select this muscle when they need a small-animal preparation for controlled investigations of skeletal-muscle structure and function. Its isolated contractile responses support studies of neuromuscular disorders, muscle injury, aging, exercise physiology, and therapeutic interventions. The model therefore connects cellular excitation and force production with broader questions about muscle health and disease.
An intervention can be examined through its effects on contractile strength, fatigue, excitation-contraction coupling, or recovery in the isolated muscle. Comparing these functional outcomes provides a way to determine whether treatment-related changes influence force generation, stimulation-linked activation, or restoration after fatigue. This makes the preparation relevant to disease, injury, and therapeutic-intervention research.