Mouse Pipetting
Mouse Pipetting - Related Videos
09:08
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
0 Views •
Cited by 59 •
2011
Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.
11:11
Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots
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Cited by 17 •
2022
The protocol describes a SARS-CoV-2 diagnostic method that utilizes open-source automation to perform RT-qPCR molecular testing of saliva samples. This scalable approach can be applied to clinical public health surveillance as well as to increase the capacity of smaller university laboratories.
07:04
A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse
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Cited by 5 •
2017
Mice are widely used to study gestational biology. However, pregnancy termination is required for such studies which precludes longitudinal investigations and necessitates the use of large numbers of animals. Therefore, we describe a non-invasive technique of high-frequency ultrasonography for early detection and monitoring of post-implantation events in the pregnant mouse.
07:46
In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
0 Views •
Cited by 39 •
2010
Orthotopic animal models of ovarian cancer replicate better human disease and therefore enhance our understanding of cancer progression and tumor response to therapy. A mouse model receives an intrabursal injection of luciferase-expressing ovarian tumor cells. Treatment is administered via oral gavage. Tumor growth is monitored by in vivo imaging system.
14:17
Nuclear Transfer into Mouse Oocytes
0 Views •
Cited by 7 •
2006
This movie and the protocol are intended to help learning nuclear transfer.
FAQs
Calibration helps the selected setting correspond to the volume actually transferred. This matters because small volume errors can alter reagent preparation, dilution steps, and the composition of samples used in assays. When the instrument is calibrated and used consistently, researchers can reduce variation between samples and strengthen the reproducibility of experiments involving mouse tissues, cells, or other biological materials.
The defined plunger stop provides a consistent point for controlling aspiration and dispensing. Pressing to the intended stop helps the operator take up and release the measured volume in a repeatable manner rather than relying on an uncontrolled movement. Consistent plunger handling supports dependable transfers into tubes, wells, and other vessels used for biological analysis.
Disposable tips help limit contamination during movement of reagents and mouse-derived materials between vessels. Using a fresh tip for the relevant transfer can help preserve sample integrity and reduce the chance that material from one tube or well affects another. This protection is important when preparing dilutions, performing cell-based assays, or analyzing biological samples.
Reproducibility depends on combining a calibrated micropipette with controlled plunger movement and consistent handling of the disposable tip. Variation in any of these elements can contribute to volume error or contamination, which may influence downstream results. Maintaining the same controlled approach across samples helps researchers compare measurements more reliably in mouse biology experiments.
Begin by selecting a calibrated micropipette and attaching a disposable tip. Set the intended volume, depress the plunger to the defined stop, and aspirate the liquid through the tip. Move the tip to the receiving tube, well, or vessel, then dispense the liquid in a controlled way. This sequence supports accurate transfer while limiting contamination.
The technique is useful whenever mouse-related studies require measured liquid handling. Examples include preparing reagents, making sample dilutions, conducting cell-based assays, and transferring mouse-derived tissues, cells, or other biological samples for analysis. These tasks occur across genetics, physiology, disease modeling, and pharmacological research, where consistent liquid volumes contribute to interpretable experimental results.
Careful pipetting can improve the reliability of results by reducing volume error, limiting contamination, and protecting sample integrity. Those improvements support more consistent reagent concentrations, dilutions, and assay conditions. In studies of mouse tissues or cells, dependable transfers make it easier to interpret measurements and compare experimental samples across biological investigations.