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Nuclear Transfer into Mouse Oocytes

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Biology

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JoVE Journal Biology

Nuclear Transfer into Mouse Oocytes

Nuclear Transfer into Mouse Oocytes

DOI:

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14:17 min

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November 30, 2006

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Dieter Egli, Kevin Eggan

¹Department of Molecular and Cell Biology,Harvard

Chapters

00:00Introduction
00:17Preparation of dishes
00:18Dish for oocyte incubation KSOM
01:08Dish for oocyte isolation HCZB and HCZB/hyaluronidase
02:23Dish for enucleation and nuclear transfer HCZB/cytocholazin
03:30Preparation of pipettes
03:31Holding pipette
04:53Injection pipette
06:30Mouth pipette for oocyte isolation
07:07Oocyte isolation
09:43Nuclear transfer
09:58Enucleation
10:31Collection of donor cells
11:05Transfer
11:50Discussion

Summary

Automatic Translation

This movie and the protocol are intended to help learning nuclear transfer.

Tags

Nuclear Transfer Mouse Oocytes Developmental Potential Epigenetic Processes Genetic Processes Regenerative Medicine Embryonic Stem Cells Cloned Preimplantation Stage Embryos Stem Cells In Vitro Study Nuclear Reprogramming Prof. Yanagimachi WAKAYAMA Et Al. 1998

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