Nuclear Transfer into Mouse Oocytes

Dieter Egli; Kevin Eggan

doi:10.3791/116

JoVE Journal
Biology

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JoVE Journal Biology

Nuclear Transfer into Mouse Oocytes

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18,499 Views

14:17 min

November 30, 2006

DOI: 10.3791/116-v

Dieter Egli¹, Kevin Eggan¹

¹Department of Molecular and Cell Biology,Harvard

Overview

This movie and the protocol are intended to help learning nuclear transfer, a process where a differentiated nucleus is reprogrammed into an embryonic state.

Key Study Components

Area of Science

Neuroscience
Cell Biology
Developmental Biology

Background

Nuclear transfer is a technique used in cloning and regenerative medicine.
Reprogramming of nuclei is crucial for understanding cellular differentiation.
This process can lead to advancements in stem cell research.
Understanding the conditions for successful nuclear transfer is essential for its application.

Purpose of Study

To demonstrate the nuclear transfer technique.
To provide a protocol that aids in the learning process.
To explore the potential for reprogramming differentiated cells.

Methods Used

Preparation of a dish with specific reagents.
Use of hyaluronidase in the nuclear transfer process.
Calibration of CO2 atmosphere for optimal conditions.
Application of mineral oil to cover drops during the procedure.

Main Results

Successful demonstration of nuclear transfer technique.
Reagents used were effective in the reprogramming process.
Optimal conditions for nuclear transfer were established.
Protocol provided can aid in further research and learning.

Conclusions

Nuclear transfer is a viable method for reprogramming nuclei.
Understanding the methodology is crucial for its application in research.
The protocol can serve as a foundation for future studies in the field.

Frequently Asked Questions

What is nuclear transfer?

Nuclear transfer is a technique used to transfer the nucleus of a differentiated cell into an enucleated egg cell, allowing for reprogramming.

Why is nuclear transfer important?

It is important for understanding cellular reprogramming and has applications in cloning and regenerative medicine.

What reagents are used in nuclear transfer?

Reagents such as SUM and HCCV, along with hyaluronidase, are commonly used in the process.

How does CO2 calibration affect nuclear transfer?

Calibrating the CO2 atmosphere is essential for maintaining optimal conditions for the cells during the nuclear transfer process.

What are the potential applications of nuclear transfer?

Potential applications include advancements in stem cell research, regenerative medicine, and cloning techniques.

This movie and the protocol are intended to help learning nuclear transfer.

So we do NU Nuclear transfer because our group is interested in reprogramming. This is a fascinating process in that the differentiated nucleus can be reprogrammed into an embryonic state. These are the reagents case SUM and HCCV and this is hyaluronidase.

And first I prepare a dish with, and this will go to the incubators to equate with a CO2. These are drops, are several drops for Turing that covered with mineral oil. So they calibrate CO2 atmosphere.

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