Mouse Retina
Mouse Retina - Related Videos
04:32
Two-Photon In Vivo Imaging of the Mouse Retina
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2025
Source: Wang, Z., et al. Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina. J. Vis. Exp. (2021).This video demonstrates a technique for in vivo retinal imaging using two-photon microscopy. Anesthetized mice are prepared for imaging by securing their heads, Applying eye lubricant, and placing a coverslip over the eye. Retinal ganglion cells (RGCs) expressing fluorescent proteins are first visualized using epifluorescence for alignment. Finally, two-photon imaging is employed,...
05:53
In vivo Electroporation of Developing Mouse Retina
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Cited by 33 •
2011
A method for the incorporation of plasmid DNA into murine retinal cells for the purpose of performing either gain- or loss of function studies in vivo is presented. This method capitalizes on the transient increase in permeability of cell plasma membranes induced by the application of an external electrical field.
07:32
Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
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Cited by 7 •
2014
The rodent retina has long been recognized as an accessible window to the brain. In this technical paper we provide a protocol that employs the mouse model of oxygen-induced retinopathy to study the mechanisms that lead to failure of vascular regeneration within the central nervous system after ischemic injury. The described system can also be harnessed to explore strategies to promote regrowth of functional blood vessels within the retina and CNS.
02:39
Evisceration of Mouse Vitreous and Retina for Proteomic Analyses
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Cited by 39 •
2011
The dissection technique illustrates evisceration of the vitreous, retina, and lens from the mouse eye, separation by centrifugation, and characterization with protein assays.
04:08
In Vivo Imaging of the Mouse Retina Using Optical Coherence Tomography
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2025
Source: Jagodzinska, J., et. al., Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo. J. Vis. Exp. (2017) In this video, we demonstrate the use of spectral-domain optical coherence tomography (SD-OCT) to image retinal layers and assess structural changes in a genetically modified mouse model with optic neuropathy.
FAQs
Layered retinal circuits distribute processing across photoreceptors, interneurons, and ganglion cells rather than passing an unprocessed signal directly to the brain. Their interactions help detect light and extract features such as contrast. This organization allows researchers to examine how cellular connections transform visual input into encoded activity before transmission through the optic nerve.
Photoreceptors initiate visual signaling when they detect light. Interneurons participate in processing the resulting information within retinal circuits, while ganglion cells transmit the processed activity through the optic nerve. Studying these cell classes together helps distinguish changes in light detection, local neural processing, and output signaling when researchers investigate normal vision or retinal disease.
Genetic and environmental changes provide ways to examine how retinal structure and function respond to altered conditions. Researchers can compare resulting effects on retinal development, neural circuitry, degeneration, or visual signaling. These comparisons help connect a specific change with abnormalities in processing or disease-related progression, while also identifying responses that may be relevant to treatment studies.
Development and degeneration address different stages of retinal biology, but both reveal how neural circuits are formed, maintained, or disrupted. Developmental studies examine the establishment of retinal organization, whereas degeneration studies focus on progressive loss or alteration of that system. Considering both contexts can clarify disease mechanisms and identify points where intervention might preserve retinal function.
Researchers use the mouse retina to examine how inherited changes influence retinal cells, circuitry, and visual signaling. Studies can focus on disease-related degeneration or altered responses within the layered neural system. This model supports evaluation of potential treatments by providing a biological setting in which researchers can investigate whether an intervention affects features associated with vision loss.
Treatment-oriented studies can assess whether genetic or environmental disease-related effects are associated with changes in retinal circuitry, degeneration, or encoded activity. The retina therefore offers several biological outcomes for examining potential benefit, rather than relying on a single feature. Such work contributes to research on inherited retinal disorders and other causes of vision loss.