Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

Journal

Biology

JoVE Journal
Biology
Author Produced

This content is Free Access.

JoVE Journal Biology

Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

DOI:

10.3791/2795-v

•

02:39 min

•

April 03, 2011

•

Jessica M. Skeie², Stephen H. Tsang, Vinit B. Mahajan²

¹Omics Laboratory,University of Iowa, ²Ophthalmology and Visual Sciences,University of Iowa, ³Harkness Eye Institute,Columbia University College of Physicians and Surgeons

Chapters

00:01Title
00:15Anterior Segment Dissection
00:38Lens and Vitreous Evisceration
01:12Retina Evisceration
01:31Centrifugation

Summary

Automatic Translation

The dissection technique illustrates evisceration of the vitreous, retina, and lens from the mouse eye, separation by centrifugation, and characterization with protein assays.

Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

•

•

•

Chapters

Summary

Automatic Translation

Tags

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Horizontal Slice Preparation of the Retina

Electrophoretic Separation of Proteins

Immunoblot Analysis

Staining Proteins in Gels

Digital Microfluidics for Automated Proteomic Processing

Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina

Trajectory Data Analyses for Pedestrian Space-time Activity Study

Read Article