Mouse Testis
Mouse Testis - Related Videos
05:24
Teratoma Generation in the Testis Capsule
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Cited by 25 •
2011
Human pluripotent stem cells (hPSCs) have the potential to treat a myriad of different diseases. The utility of these cells lies in the fact that they can differentiate into any cell type in the body. Here we describe the teratoma assay, which is used to demonstrate the pluripotence of hPSCs.
10:41
Germ Cell Transplantation and Testis Tissue Xenografting in Mice
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Cited by 16 •
2012
Protocols for germ cell transplantation and testis tissue xenografting are described. Germ cell transplantation results in donor-derived spermatogenesis in recipient testes and represents a functional reconstitution assay for identification of spermatogonial stem cells (SSCs). Testis tissue xenografting reproduces testis development and spermatogenesis of various donor species in recipient mice.
08:39
In Vivo Microinjection and Electroporation of Mouse Testis
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Cited by 14 •
2014
This article describes microinjection and electroporation of mouse testis in vivo as a transfection technique for testicular mouse cells to study unique processes of spermatogenesis. The presented protocol involves steps of glass capillary preparation, microinjection via the efferent duct, and transfection by electroporation.
14:32
Polysome Profiling in Leishmania, Human Cells and Mouse Testis
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Cited by 21 •
2018
The overall goal of polysome profiling technique is analysis of translational activity of individual mRNAs or transcriptome mRNAs during protein synthesis. The method is important for studies of protein synthesis regulation, translation activation and repression in health and multiple human diseases.
05:48
An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity
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Cited by 7 •
2018
Here, we present a protocol to assess the blood-testis barrier integrity by injecting inulin-FITC into testes. This is an efficient in vivo method to study blood-testis barrier integrity that can be compromised by genetic and environmental elements.
FAQs
Sertoli cells remain closely associated with germ cells within the seminiferous tubules, where spermatogenesis takes place. This cellular arrangement makes them central to studies of germ-cell development and testicular function. By examining these relationships, researchers can investigate how changes affecting the tubules may influence reproductive processes and contribute to problems with fertility.
Leydig cells occupy the tissue surrounding the seminiferous tubules and synthesize testosterone in response to hormonal signals. Their activity provides an endocrine dimension to testis research that is distinct from germ-cell development. Investigators can therefore examine how hormonal regulation relates to overall testicular function, fertility, and disorders involving male reproductive physiology.
The organization separates two closely related areas of investigation. Germ cells and Sertoli cells within seminiferous tubules support analysis of spermatogenesis, whereas Leydig cells in surrounding tissue support analysis of testosterone production and endocrine regulation. Considering both compartments helps researchers interpret how cellular changes may affect testicular function and reproductive outcomes.
Examination after a genetic change can reveal effects on germ-cell development, testicular function, endocrine regulation, and fertility. The mouse testis is especially useful for connecting an altered gene with changes in reproductive biology rather than evaluating a single outcome alone. Such studies may also clarify mechanisms that contribute to male infertility.
Mouse testes provide a system for investigating reproductive toxicology, including how environmental exposures may affect germ-cell development, testicular function, hormone-related regulation, or fertility. Researchers can use this model to connect an exposure with changes in mammalian reproductive processes. The resulting evidence supports analysis of potential mechanisms underlying reproductive disorders and impaired male fertility.
Cellular observations can link abnormalities in germ-cell development or seminiferous-tubule organization with broader effects on fertility. At the same time, examining Leydig cells and testosterone production adds information about endocrine regulation. Combining these perspectives helps researchers study male infertility as a condition involving both reproductive-cell processes and hormonal control, rather than only one cellular type.